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Calcium-Binding Protein Protocols

Volume 2: Methods and Techniques

Series: Methods in Molecular Biology

by Hans J. Vogel

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Main description:

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on-off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

Contents:

II. Methods to Study Calcium-Binding Proteins

Quantitative Analysis of Ca2+ Binding by Flow Dialysis
Michio Yazawa

Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators
Sara Linse

Deconvolution of Calcium Binding Curves: Facts and Fiction
Jacques Haiech and Marie-Claude Kilhoffer

Absorption and Circular Dichroism Spectroscopy
Stephen R. Martin and Peter M. Bayley

Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins
Heinz Fabian and Hans J. Vogel

Steady State Fluorescence Spectroscopy
Aalim M. Weljie and Hans J. Vogel

Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins
J. David Johnson and Svetlana B. Tikunova

Surface Plasmon Resonance of Calcium-Binding Proteins
Karin Julenius

Differential Scanning Calorimetry
Maria M. Lopez and George I. Makhatadze

Isothermal Titration Calorimetry
Maria M. Lopez and George I. Makhatadze

Multiangle Laser Light Scattering and Sedimentation Equilibrium
Leslie D. Hicks, Jean-Rene Alattia, Mitsuhiko Ikura, and Cyril M. Kay

Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in Their Interactions with Regulatory Targets
Jill Trewhella and Joanna K. Krueger

Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry
Amanda L. Doherty-Kirby and Gilles A. Lajoie

Synthetic Calcium-Binding Peptides
Gary S. Shaw

Proteolytic Fragments of Calcium-Binding Proteins
Richard D. Brokx and Hans J. Vogel

Electron Magnetic Resonance Studies of Calcium-Binding Proteins
Lawrence J. Berliner

Cadmium-113 and Lead-207 NMR of Calcium-Binding Proteins
Teresa E. Clarke and Hans J. Vogel

Calcium-43 of NMR ofCalcium-Binding Proteins
Torbjoern Drakenberg

Exploring Familial Relationships Using Multiple Sequence Alignment
Aalim M. Weljie and Jaap Heringa

Structure Determination by NMR - Isotope Labeling
Monica X. Li, David C. Corson, and Brian D. Sykes

Protein Structure Calculation from NMR Data
Tapas K. Mal, Stefan Bagby, and Mitsuhiko Ikura

Shape and Dynamics of Calcium Binding Proteins Investigated by Nitrogen-15 NMR Relaxation
Joern M. Werner, Iain D. Campbell, and A. Kristina Downing

The Use of Dipolar Couplings for the NMR Structure Refinement of a Pair of Calcium Binding EGF Domains
Jonathan Boyd, Iain D. Campbell, and A. Kristina Downing

Vector Geometry Mapping: A Method to Characterise the Conformation of Helix-Loop-Helix Calcium Binding Proteins
Kyoko L. Yap, James B. Ames, Mark B. Swindells, and Mitsuhiko Ikura

Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography
Ryoji Kobayashi

Enzymatic Assays to Compare Calmodulin Isoforms, Mutants and Chimeras
Michael P. Walsh, Jacquelyn E. Van Lierop, Cindy Sutherland, Ritsu Kondo, and J. David Johnson

Gene Expression in Transfected Cells
Kate Hughes, Juha Saarikettu, and Thomas Grundstroem

Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins
Anthony Persechini

Studying the Spatial Distribution of Ca2+-Binding Proteins: How Does it Work for Calmodulin?
Katalin Toeroek, Richard Thorogate, and Steven Howell

PRODUCT DETAILS

ISBN-13: 9781617371363
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 436
Weight: 637g
Availability: Available
Subcategories: Biochemistry

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