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Immunotherapy of Cancer

Methods and Protocols

Series: Methods in Molecular Biology

by Patricia Yotnda

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Main description:

A continuously evolving technique, immunotherapy for the treatment of cancers now incorporates the use of immune cells infused during bone marrow transplants as well as approaches like cell and gene therapy, while stem cell-based therapies, tissue engineering, and targeting have also contributed to the latest successes in pre-clinical immunotherapy studies. In Immunotherapy of Cancer: Methods and Protocols, expert researchers in the field describe detailed procedures for trainees and experts in the area of basic, clinical science who wish to undertake their own cutting-edge immunotherapy studies. In addition to the protocols, the volume also contains two general overviews providing useful updates in each area as well as summaries of recent pre-clinical and clinical trials. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Immunotherapy of Cancer: Methods and Protocols seeks to guide scientists along the path to a further developed system of immunotherapeutic treatments in order to reduce and hopefully eradicate this terrible disease.

Contents:

1. Hypoxic Tumors and Their Effect on Immune Cells and Cancer Therapy
Patricia Yotnda, Danli Wu, and Anna May Swanson

2. Characterization of Regulatory T Cells in Tumor Suppressive Microenvironments
Guangyong Peng

3. Generation of Cytotoxic T Lymphocytes for Immunotherapy of EBV-Associated Malignancies
Corey Smith and Rajiv Khanna

4. Acquisition, Preparation, and Functional Assessment of Human NK Cells for Adoptive Immunotherapy
Dean A. Lee, Michael R. Verneris, and Dario Campana

5. Enhanced Migration of Human Dendritic Cells Expressing Inducible CD40
Natalia Lapteva

6. Differentiation of Human Embryonic Stem Cells into Hematopoietic Cells in vitro
Eun-Mi Kim and Nicholas Zavazava

7. Gene Therapy to Improve Migration of T Cells to the Tumor Site
Antonio Di Stasi, Biagio De Angelis, and Barbara Savoldo

8. Gene Therapy to Improve Function of T Cells for Adoptive Immunotherapy
Concetta Quintarelli, Barbara Savoldo, and Gianpietro Dotti

9. Cytokine-FC Fusion Genes as Molecular Adjuvants for DNA Vaccines
Daniel Hirschhorn-Cymerman and Miguel-Angel Perales

10. Pharmacology of Anti-CD3 Diphtheria Immunotoxin in CD3 Positive T Cell Lymphoma Trials
Yu-Jen Lee, Jung Hee Woo, David M. Neville, and Arthur E. Frankel

11. Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display
Thomas Schirrmann and Michael Hust

12. Identification of Immunogenic Peptides of the Self-Tumor Antigen: Our Experience with Telomerase Reverse Transcriptase
Xochitl Cortez-Gonzalez and Maurizio Zanetti

13. Rescue, Amplification, Purification, and PEGylation of Replication Defective First-Generation Adenoviral Vectors
Michael A. Barry, Eric A. Weaver, and S.E. Hofherr

14. Adenovirus-Mediated Interleukin (IL)-24 Immunotherapy for Cancer
Rajagopal Ramesh, Constantine G. Ioannides, Jack A. Roth, and Sunil Chada

15. Liposomes for Gene Transfer in Cancer Therapy
Nancy Smyth Templeton

16. Oncolytic HSV as a Vector in Cancer Immunotherapy
Hongtao Li and Xiaoliu Zhang

17. Generation of Chimeric T Cell Receptor Transgenes and Their Efficient Transfer in Primary Mouse T Lymphocytes
Linda J. Berry, Nicole M. Haynes, and Phillip K. Darcy

18. Multi-Walled Carbon Nanotube (MWCNT) Synthesis, Preparation, Labeling, and Functionalization
Babak Kateb, Vicky Yamamoto, Darya Alizadeh, Leying Zhang, Harish M. Manohara, Michael J. Bronikowski, and Behnam Badie

19. Cellular Immunotherapy of Cancer
Fatma Okur and Malcolm K. Brenner

PRODUCT DETAILS

ISBN-13: 9781607617853
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2010
Pages: 368
Weight: 1890g
Availability: Available
Subcategories: Immunology, Oncology

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