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Glycosylation Engineering of Biopharmaceuticals

Methods and Protocols

Series: Methods in Molecular Biology

by Alain Beck and Alan Beck

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Glycosylation Engineering of Biopharmaceuticals

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Main description:

Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins. In Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols, experts in the field provide readers with production and characterization protocols of glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. The volume is divided in four complementary parts dealing with glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complexes characterization, and PK/PD assays for therapeutic antibodies. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols serves as an ideal guide for scientists striving to push forward the exciting field of engineered biopharmaceuticals.

Contents:

Part I: Glyco-Engineering of Therapeutic Proteins

1. Engineering of Therapeutic and Diagnostic O-Glycans on Recombinant Mucin-Type Immunoglobulin Fusion Proteins Expressed in CHO Cells

Linda Lindberg, Jining Liu, and Jan Holgersson

2. Engineering a Human-Like Glycosylation to Produce Therapeutic Glycoproteins Based on 6-Linked Sialylation in CHO Cells

Nassimal El Mai, Sandrine Donadio-Andrei, Chloe Iss, Valerie Calabro, and Catherine Ronin

3. Glycoengineered Pichia-Based Expression of Monoclonal Antibodies

Dongxing Zha

4. N-Glycosylation Humanization for Production of Therapeutic Recombinant Glycoproteins in Saccharomyces cerevisiae

Christelle Arico, Christine Bonnet, and Christophe Javaud

5. Engineering the Baculovirus Genome to Produce Galactosylated Antibodies in Lepidopteran Cells

Sylvie Juliant, Marylene Leveque, Pierre Cerutti, Annick Ozil, Sylvie Choblet, Marie-Luce Violet, Marie-Christine Slomianny, Anne Harduin-Lepers, and Martine Cerutti

Part II: Glycoanalytics

6. Nano LC Chips MS/MS for the Characterization of N-Glycopeptides Generated from Trypsin Digestion of a Monoclonal Antibody

Elsa Wagner-Rousset, Christine Schaeffer-Reiss, Audrey Bednarczyk, Nathalie Corvaia, Alain Van Dorsselaer, and Alain Beck

7. Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry

Marie-Claire Janin-Bussat, Laure Tonini, Celine Huillet, Olivier Colas, Christine Klinguer-Hamour, Nathalie Corvaia, and Alain Beck

8. Therapeutic Antibody Glycosylation Analysis: A Contract Research Organization Perspective in the Frame of Batch Release or Comparability Support

Arnaud Delobel, Fabrice Cantais, Anicet Catrain, Erell Dereux, and Gery Van Vyncht

9. Mass Spectrometric Analysis of O-Linked Oligosaccharides from Various Recombinant Expression Systems

Diarmuid T. Kenny, Stefan Gaunit, Catherine A. Hayes, Anki Gustafsson, Magnus Sjoeblom, Jan Holgersson, and Niclas G. Karlsson

10. Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC

T. Shantha Raju

11. Application of Capillary Electrophoresis in Glycoprotein Analysis

Richard R. Rustandi, Carrie Anderson, and Melissa Hamm

12. Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology

Grace Chen, Sha Ha, and Richard R. Rustandi

13. Hydrophobic Interaction Chromatography to Analyze Glycoproteins

Richard R. Rustandi

14. Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7

Ludovic Landemarre and Eric Duverger

15. Analysis of Monoclonal Antibodies by Sedimentation Velocity Analytical Ultracentrifugation

W. Blaine Stine, Jr.

Part III: Glycoprotein Complexes Characterization

16. Noncovalent Mass Spectrometry for the Characterization of Antibody/Antigen Complexes

Cedric Atmanene, Elsa Wagner-Rousset, Nathalie Corvaia, Alain Van Dorsselaer, Alain Beck, and Sarah Sanglier-Cianferani

17. Conformational Analysis of Recombinant Monoclonal Antibodies with Hydrogen/Deuterium Exchange Mass Spectrometry

Damian Houde and John R. Engen

18. Epitope Mapping of Antibodies by Mass Spectroscopy: A Case Study

Victor H. Obungu, Valentina Gelfanova, and Lihua Huang

Part IV: PK/PD Assays for Therapeutic Antibodies

19. Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Deshydrogenase (LDH) Measurement

Matthieu Broussas, Lucile Broyer, and Liliane Goetsch

20. Evaluation of Complement Dependent Cytotoxicity Using ATP Measurement and C1q / C4b Binding

Lucile Broyer, Liliane Goetsch, and Mattthieu Broussas

21. Capture of the Human IgG1 Antibodies by Protein A for the Kinetic Study of h-IgG / FcgR Interaction Using SPR-Based Biosensor Technology

Thierry Champion and Alain Beck

22. Mass Spectrometry Protocol for the Absolute Quantification of a Monoclonal Antibody in Serum with Immunopurification

Francois Becher, Mathieu Dubois, Francois Fenaille, and Eric Ezan

PRODUCT DETAILS

ISBN-13: 9781627033268
Publisher: Springer (Humana Press Inc.)
Publication date: March, 2013
Pages: 370
Weight: 8416g
Availability: Available
Subcategories: Pharmacology

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