Wisepress Medical Bookshop

Cancer Susceptibility

Methods and Protocols

Series: Methods in Molecular Biology

by Michelle Webb

Price

Quantity

€191.99
(To see other currencies, click on price)

Hardback

Add to basket

Overview

Products Details

Customer Reviews

MORE ABOUT THIS BOOK

Main description:

Over the past two decades, spectacular advances have been made in our understanding of the molecular genetics of cancer, leading to the pursuit of identifying genes that, when mutated, result in an increased susceptibility to the disease. In Cancer Susceptibility: Methods and Protocols, experts in the field bring together the most recent technological developments for identifying and screening cancer susceptibility genes. Divided into two clear sections, the book begins with gene identification, which updates and informs scientists working at identifying novel cancer susceptibility genes, while the second part deals with mutation screening technologies that aid scientists and clinicians working to translate this knowledge into the clinic. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cancer Susceptibility: Methods and Protocols is a timely collection that seeks to provide researchers with the tools to predict and combat this terrible disease.

Contents:

Part I: Identifying Cancer Susceptibility Genes

1. The Identification of Colon Cancer Susceptibility Genes by Using Genome-Wide Scans
Denise Daley

2. Prioritizing Candidate Genetic Modifiers of BRCA1 and BRCA2 Using a Combinatorial Analysis of Global Expression and Polymorphism Association Studies of Breast Cancer
Logan C. Walker and Amanda B. Spurdle

3. Microarray Based Comparative Genomic Hybridization (Array-CGH) as a Useful Tool for Identifying Genes Involved in Glioblastoma (GBM)
Yolanda Ruano, Manuela Mollejo, Angel Rodriguez de Lope, Jose Luis Hernandez-Moneo, Pedro Martinez, and Barbara Melendez

4. Multiplex Amplifiable Probe Hybridization (MAPH) Methodology as an Alternative to Comparative Genomic Hybridization (CGH)
Ludmila Kousoulidou, Carolina Sismani, and Philippos C. Patsalis

5. Utilizing Saccharomyces cerevisiae to Identify Aneuploidy and Cancer Susceptibility Genes
Erin D. Strome and Sharon E. Plon

6. Computational Identification of Cancer Susceptibility Loci
Marko Laakso, Sirkku Karinen, Rainer Lehtonen, and Sampsa Hautaniemi

7. Digital Candidate Gene Approach (DigiCGA) for Identification of Cancer Genes
Meng-Jin Zhu, Xiang Li, and Shu-Hong Zhao

Part II: Screening Cancer Susceptibility Genes

8. The Use of Denaturing High Perfomance Liquid Chromatography (DHPLC) For Mutation Scanning of Hereditary Cancer Genes
Deborah J. Marsh PhD and Viive M. Howell

9. Enhanced Mismatch Mutation Analysis: Simultaneous Detection of Point Mutations and Large Scale Rearrangements by Capillary Electrophoresis, Application to BRCA1 and BRCA2
Claude Houdayer, Virginie Moncoutier, Jerome Champ, Jeremie Weber, Jean-Louis Viovy, and Dominique Stoppa-Lyonnet

10. Economical Protocol for Combined Single-Strand Conformation Polymorphism and Heteroduplex Analysis on a Standard Capillary Electrophoresis Apparatus
Piotr Kozlowski and Wlodzimierz J. Krzyzosiak

11. Mutational Screening of hMLH1 and hMSH2 that Confer Inherited Colorectal Cancer Susceptibility Using Denature Gradient Gel Electrophoresis (DGGE)
Tao Liu

12. s-RT-MELT: A Novel Technology for Mutation Screening
Jin Li and G. Mike Makrigiorgos

13. Zoom-In Array Comparative Genomic Hybridization (aCGH) to Detect Germline Rearrangements in Cancer Susceptibility Genes
Johan Staaf and Ake Borg

14. Development of a Scoring System to Screen for BRCA1/2 Mutations
Gareth R. Evans and Fiona Lalloo

15. Use of Splicing Reporter Minigene Assay to Evaluate the Effect on Splicing of Unclassified Genetic Variants
Pascaline Gaildrat, Audrey Killian, Alexandra Martins, Isabelle Tournier, Thierry Frebourg, and Mario Tosi

16. Functional Analysis of Human BRCA2 Variants Using a Mouse Embryonic Stem Cell-Based Assay
Sergey G. Kuznetsov, Suhwan Chang, and Shyam K. Sharan

17. Developing Functional Assays for BRCA1 Unclassified Variants
Michelle Webb

PRODUCT DETAILS

ISBN-13: 9781607617587
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2010
Pages: 306
Weight: 850g
Availability: Available
Subcategories: Genetics, Oncology

Related books
From the same series

CUSTOMER REVIEWS

Average Rating