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MORE ABOUT THIS BOOK
Main description:
​This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Feature:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents:
1. Xanthomonas and the TAL effectors:Â Nature's Molecular Biologist
Frank White
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2. TAL Effector DNA-binding Principles and Specificity
Annekatrin Richter, Jana Streubel, and Jens Boch
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3. The Development of TALE Nucleases for Biotechnology
David G. Ousterout and Charles A. Gersbach
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4. Online Tools for TALEN Design
Adam J. Bogdanove and Nicholas J. Booher
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5. Assembly of Customized TAL Effectors through Advanced ULtiMATE System
Junjiao Yang, Shengjie Guo, Pengfei Yuan, and Wensheng Wei
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6. Engineering Customized TALENs Using the Platinum Gate TALEN Kit
Tetsushi Sakuma and Takashi Yamamoto
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7. Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors
Pratiksha I. Thakore and Charles A. Gersbach
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8. A New Approach to Dissect Nuclear Organization; TALE-mediated Genome Visualization (TGV)
Yusuke Miyanari
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9. TALEN Induced Translocations in Human Cells
Marion Piganeau, Benjamin Renouf, Hind Ghezraoui, and Erika Brunet
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10. Mutagenesis in Newts; Protocol for Iberian Ribbed Newts
Toshinori Hayashi and Takashi Takeuchi
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11. Targeted Mutagenesis in Bombyx mori Using TALENs
Yoko Takasu, Toshiki Tamura, Marian Goldsmith, and Michal Zurovec
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12. GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, The TwoSpotted Cricket Gryllus bimaculatus, using TALENs
Takahito Watanabe, Sumihare Noji, Â and Taro Mito
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13. Methods for TALEN Evaluation, use, and Mutation Detection in the Mosquito Aedes aegypti
Sanjay Basu, Azadeh Aryan, Mary Etna Haac, Kevin M. Myles, and Zach N. Adelman
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14. Methods for TALEN-mediated Genomic Manipulations in Drosophila
Jiyong Liu, Yawen Guo, Changqing Li, Yixu Chen, and Renjie Jiao
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15. Targeted Mutagenesis in Zebrafish by TALENs
Peng Huang, An Xiao, Xiangjun Tong, Shuo Lin, and Bo Zhang
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16. Mutagenesis in Xenopus and Zebrafish using TALENs
Yun Liu, Hui Zhao, and Christopher H.K. Cheng
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17. Genome Editing in Mice using TALE Nucleases
Benedikt Wefers, Christina Brandl, Oskar Ortiz, Wolfgang Wurst, and Ralf Kühn
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18. Genome Editing in Rats using TALE Nucleases
Laurent Tesson, Séverine Remy, Séverine Ménoret, Claire Usal, Reynald Thinard, Chloé Savignard, Anne De Cian, Carine Giovannangeli, Jean-Paul Concordet, and Ignacio Anegon
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19 Designer Nuclease-mediated Generation of Knock out THP1 Cells
Tobias Schmidt, Jonathan L. Schmid-Burgk, Thomas S. Ebert, Moritz M. Gaidt, and Veit Hornung
PRODUCT DETAILS
Publisher: Springer (Springer New York)
Publication date: October, 2015
Pages: 265
Weight: 817g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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