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MORE ABOUT THIS BOOK
Main description:
Baculovirus Expression Protocols, Second Edition, provides the detailed steps required to perform the techniques involved with the use of baculoviruses and insect cell culture and discusses problems that may be encountered. This newly-revised and updated edition provides a cutting edge step-by-step guide for biochemists, molecular biologists, biochemical engineers and others using the BEVS and/or insect cells for producing recombinant proteins. Furthermore, Baculovirus Expression Protocols, Second Edition, provides assistance to scientists and engineers interested in developing and producing baculovirus insecticides. Authors list all available insect cell lines and provide methods for isolating new cell lines. The procedures involved in producing products, both at laboratory scale and large scale, are discussed, as well as the production in insect larvae. It is hoped that this volume will not only aid the user in successfully completing the tasks described herein, but will also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture. Timely and thorough, this volume will become an essential reference for anyone using baculoviruses and insect cell culture.
Contents:
Table of Contents
PART I. INTRODUCTION
Chapter 1 Useful Tips, Widely Used Techniques and Quantifying Cell Metabolic Behavior
David W. Murhammer
PART II. BACULOVIRUS MOLECULAR BIOLOGY/DEVELOPMENT OF RECOMBINANT BACULOVIRUSES
Chapter 2 Introduction to Baculovirus Molecular Biology
Barbara J. Kelly, Linda A. King, and Robert D. Possee
Chapter 3 Baculovirus Transfer Vectors
Robert D. Possee and Linda A. King
Chapter 4 Recombinant Baculovirus Isolation
Linda A. King, Richard Hitchman, and Robert D. Possee
Chapter 5 Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System
James A. Fornwald, Quinn Lu, Dayuan Wang, and Robert S. Ames
PART III. INSECT CELL CULTURE
Chapter 6 Available Lepidopteran Insect Cell Lines
Dwight E. Lynn
Chapter 7 Lepidopteran Insect Cell Line Isolation From Insect Tissue
Dwight E. Lynn
Chapter 8 Development of Serum-Free Media for Lepidopteran Insect Cell Lines
Sprios N. Agathos
Chapter 9 Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses
Dwight E. Lynn
PART IV. PROTEIN PRODUCTION WITH RECOMBINANT BACULOVIRUSES
Chapter 10 Small Scale Protein Production With the Baculovirus Expression Vector System
Steve Harwood
Chapter 11 Recombinant Protein Production in Large Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
Cynthia B. Elias, Barbara Jardin, and Amine Kamen
Chapter 12 Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors
Sue H. Kadwell and Philip I. Hardwicke
Chapter 13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae
Yi Liu, Nathan DeCarolis, and Nikolai van Beek
Chapter 14 Virus-Like Particle and Viral Vector Production Using the BEVS/Insect Cell System: Adeno-Associated Virus Based Products
Marc G. Aucoin, Danielle Jacob, Parminder S. Chahal, Jamal Meghrous, Alice Bernier, and Amine A. Kamen
PART V. RECOMBINANT PROTEIN PRODUCTION WITH TRANSFORMED INSECT CELLS
Chapter 15 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
Robert L. Harrison and Donald L Jarvis
Chapter 16 Transformation of Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
Lucy Cherbas and Peter Cherbas
Chapter 17 Transforming Lepidopteran Insect Cells for Improved Protein Processing
Robert L. Harrison and Donald L Jarvis
PART VI. BACULOVIRUS DEVELOPMENT AND PRODUCTION FOR USE AS INSECTICIDES
Chapter 18 Introduction to the Use of Baculoviruses as Biological Insecticides
Bryony C. Bonning and Tyasning Nusawardani
Chapter 19 Baculovirus Insecticide Production in Insect Larvae
Nikolai van Beek and David C. Davis
Chapter 20 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses
Huarong Li and Bryony C. Bonning
PART VII. MISCELLANEOUS TECHNIQUES AND APPLICATIONS OF THE BACULOVIRUS/INSECT CELL SYSTEM
Chapter 21 Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy
Hyung Joon Cha and William E. Bentley
Chapter 22 Alternative Molecular and Reactor Strategies Probing Infection and Production
Yu-Chen Hu and William E. Bentley
Chapter 23 Methods For Gene Silencing with RNAi
John C. March and William E. Bentley
Chapter 24 Use of the Baculovirus/Insect Cell System to Study Apoptosis
Nor Chejanovsky
Chapter 25 Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells
Christian Hofmann
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 486
Weight: 709g
Availability: Available
Subcategories: Biochemistry
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