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Main description:
As the mysteries stored in our DNA have been more completely revealed, scientists have begun to face the extraordinary challenge of unraveling the int- cate network of protein-protein interactions established by that DNA fra- work. It is increasingly clear that proteins continuously interact with one another in a highly regulated fashion to determine cell fate, such as proliferation, diff- entiation, or death. These protein-protein interactions enable and exert str- gent control over DNA replication, RNA transcription, protein translation, macromolecular assembly and degradation, and signal transduction; essentially all cellular functions involve protein-protein interactions. Thus, protein-p- tein interactions are fundamental for normal physiology in all organisms. Alt- ation of critical protein-protein interactions is thought to be involved in the development of many diseases, such as neurodegenerative disorders, cancers, and infectious diseases. Therefore, examination of when and how protein-p- tein interactions occur and how they are controlled is essential for understa- ing diverse biological processes as well as for elucidating the molecular basis of diseases and identifying potential targets for therapeutic interventions. Over the years, many innovative biochemical, biophysical, genetic, and computational approaches have been developed to detect and analyze p- tein-protein interactions. This multitude of techniques is mandated by the diversity of physical and chemical properties of proteins and the sensitivity of protein-protein interactions to cellular conditions.
Contents:
Part I. Overview
Structural Basis of Protein-Protein Interactions
Robert C. Liddington
Quantitative Analysis of Protein-Protein Interactions
Keith D. Wilkinson
Part II. In Vitro Techniques
Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry
Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freire
Circular Dichroism Analysis for Protein-Protein Interactions
Norma J. Greenfield
Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
Guanghua Gao, Jason G. Williams, and Sharon L. Campbell
Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance
John Northup
Using Light Scattering to Determine the Stoichiometry of Protein Complexes
Jeremy Mogridge
Sedimentation Equilibrium Studies
Ian A. Taylor, John F. Eccleston, and Katrin Rittinger
Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography
Rosemarie Wilton, Elizabeth A. Myatt, and Fred J. Stevens
Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions
Sang-Hyun Park and Ronald T. Raines
Fluorescence Polarization Assay to Quantify Protein-Protein Interactions
Sang-Hyun Park and Ronald T. Raines
Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot
Randy A. Hall
Glutathione-S-Transferase-Fusion-Based Assays for Studying Protein-Protein Interactions
Haris G. Vikis and Kun-Liang Guan
Affinity Capillary Electrophoresis Analyses of Protein-Protein Interactions in Target-Directed Drug Discovery
William E. Pierceall, Lixin Zhang, and Dallas E. Hughes
Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting
Nick Loizos
Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions
Michael Mourez and R. John Collier
Part III. Detecting Protein-Protein Interactions in Heterologous Systems
A Bacterial Two-Hybrid System Based on Transcriptional Activation
Simon L. Dove and Ann Hochschild
Using the Yeast Two-Hybrid System to Identify Interacting Proteins
John Miller and Igor Stagljar
Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System
Ilya G. Serebriiskii and Elena Kotova
The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
Safia Thaminy, John Miller, and Igor Stagljar
Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae
Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn
Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo
Jae Woon Lee and Soo-Kyung Lee
Co-Immunoprecipitation from Transfected Cells
Shane C. Masters
Part IV: Probing Protein-Protein Interactions in Living Cells
Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET)
Brian Herman, R. V. Krishnan, and Victoria E. Centonze
Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer
Francis Ka-Ming Chan
Fluorescence Correlation Spectroscopy: A New Tool for Quantification of Molecular Interactions
Keith M. Berland
Confocal Microscopy for Intracellular Co-localization of Proteins
Toshiyuki Miyashita
Mapping Biochemical Networks with Protein-Fragment Complementation Assays
Ingrid Remy and Stephen W. Michnick
In Vivo Protein Cross-Linking
Fabrice Agou, Fei Ye, and Michel Veron
Part V. Proteomics-Based Approaches
Computational Prediction of Protein-Protein Interactions
John C. Obenauer and Michael B. Yaffe
Affinity Methods for
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 550
Weight: 920g
Availability: Available
Subcategories: Biochemistry
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