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Expressed Sequence Tags (ESTs)

Generation and Analysis

Series: Methods in Molecular Biology

by John Parkinson

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Main description:

Over the past two decades, expressed sequence tags (ESTs - single pass reads from randomly selected cDNAs), have proven to be a remarkably cost-effective route for the purposes of gene discovery. Gaining in popularity, millions of ESTs have now been generated for over a thousand different species. In Expressed Sequence Tags (ESTs): Generation and Analysis, leading experts in the field introduce the reader to many of the fundamental concepts underlying the generation and analysis of ESTs through readily accessible and affordable sequencing technologies. The volume focuses on various methods used to generate, process and analyze EST datasets, while also exploring the use of EST technology for other purposes such as expression profiling, analysis of alternative transcripts, and phylogenomics. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and programs, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Expressed Sequence Tags (ESTs): Generation and Analysis serves as an ideal reference for scientists continuing the vital investigation of genes and the genome.

Contents:

1. Expressed Sequence Tags: An Overview John Parkinson and Mark Blaxter 2. Strategies for Undertaking Expressed Sequence Tag (EST) Projects Sandra W. Clifton and Makedonka Mitreva 3. Construction of cDNA Libraries: Focus on Protists and Fungi Naiara Rodriguez-Ezpeleta, Shona Teijeiro, Lise Forget, Gertraud Burger and B. Franz Lang 4. Generation of Full-Length cDNA Libraries: Focus on Plants Motoaki Seki, Asako Kamiya, Piero Carninci, Yoshihide Hayashizaki, and Kazuo Shinozaki 5. Preparation of Full-Length cDNA Libraries: Focus on Metazoans Masako Harada and Yoshihide Hayashizaki 6. Expressed Sequence Tags: Normalization and Subtraction of cDNA Libraries Marcelo Bento Soares, Maria de Fatima Bonaldo, Jeremiah D. Hackett, and Debashish Bhattacharya 7. Generating EST Libraries: Trans-Spliced cDNAs Cecilia Fernandez and Rick M. Maizels 8. Large-Scale Sequencing and Analytical Processing of ESTs Makedonka Mitreva and Elaine R. Mardis 9. EST Processing: From Trace to Sequence Ralf Schmid and Mark Blaxter 10. Obtaining Accurate Translations from Expressed Sequence Tags James Wasmuth and Mark Blaxter 11. EST Databases and Web Tools for EST Projects Yao-Qing Shen, Emmet O'Brien, Liisa Koski, B. Franz Lang, and Gertraud Burger 12. Phylogenomic Analysis of EST Datasets Jose M. Peregrin-Alvarez and John Parkinson 13. Statistical Analysis of Expressed Sequence Tags Edward Susko and Andrew J. Roger 14. Application of ESTs in Microarray Analysis Weizhong Li, Lisa Olohan, Daryl Williams, Margaret Hughes, Andrew Gracey, and Andrew Cossins 15. Exploiting ESTs in Human Health Sandro Jose de Souza

PRODUCT DETAILS

ISBN-13: 9781617377600
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 342
Weight: 848g
Availability: Available
Subcategories: Biochemistry, Genetics

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