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Neutrophil Methods and Protocols

Series: Methods in Molecular Biology

by Mark T. Quinn, Frank R. DeLeo and Gary M. Bokoch

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Main description:

This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.

Contents:

Neutrophils

Methods and Protocols

Edited by

Mark T. Quinn
Frank R. DeLeo
Gary M. Bokoch
Contents

Dedication i
Preface ii
Contributors v

PART I: NEUTROPHILS: AN OVERVIEW

1. The Role of Neutrophils in the Immune System: An Overview
Harry L. Malech

PART II: NEUTROPHIL ISOLATION AND SUBCELLULAR FRACTIONATION

2. Isolation of Human Neutrophils From Venous Blood
William M. Nauseef

3. Neutrophil Isolation From Non-Human Species
Daniel W. Siemsen, Igor A. Schepetkin, Lilia N. Kirpotina, Benfang Lei, and Mark T. Quinn

4. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
Lene Udby and Niels Borregaard

PART III. BIOCHEMISTRY, BIOLOGY AND SIGNAL TRANSDUCTION OF NEUTROPHILS

5. Rac and Rap GTPase Activation Assays
Ulla G. Knaus, Alison Bamberg, and Gary M. Bokoch

6. Measurement of Phospholipid Metabolism in Intact Neutrophils
Susan Sergeant and Linda C. McPhail

7. Analysis of Protein Phosphorylation in Human Neutrophils
Jamel El-Benna and Pham My-Chan Dang

8. Mitogen-Activated Protein Kinase Assays
Martine Torres

9. Transduction of Proteins into Intact Neutrophils
Tieming Zhao and Gary M. Bokoch

10. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils
Esther J. Hillson, Sharon Dewitt, and Maurice B. Hallett

11. Analysis of Electrophysiological Properties and Responses of Neutrophils
Deri Morgan and Thomas E. DeCoursey

12. Analysis of Neutrophil Apoptosis
Emma L. Taylor, Adriano G. Rossi, Ian Dransfield, and Simon P. Hart

PART IV. NEUTROPHIL ADHESION AND CHEMOTAXIS

13. Optical and Fluorescence Detection of Neutrophil Integrin Activation
Ulrich Y. Schaff, Melissa R. Sarantos, Harold Ting, and Scott I. Simon

14. Analysis of Neutrophil Polarization and Chemotaxis
Mary A. Lokuta, Paul A. Nuzzi, and Anna Huttenlocher

15. Quantifying and Localizing Actin Free Barbed Ends in Neutrophils
Michael Glogauer

16. Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow
Mark A. Jutila, Bruce Walcheck, Robert Bargatze, and Aiyappa Palecanda

17. Model Systems to Investigate Neutrophil Adhesion and Chemotaxis
Nancy A. Louis, Eric Campbell, and Sean P. Colgan

PART V. NEUTROPHIL PHAGOCYTOSIS AND BACTERICIDAL ACTIVITY

18. Immunofluorescence and Confocal Microscopy of Neutrophils
Lee-Ann H. Allen

19. Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy
Benjamin E. Steinberg and Sergio Grinstein

20. Analysis of Neutrophil Membrane Traffic During Phagocytosis
Per Loennbro, Pontus Nordenfelt, and Hans Tapper

21. Analysis of Neutrophil Bactericidal Activity
Jessie N. Green, Christine C. Winterbourn, and Mark B. Hampton

22. A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions
Per Follin and Claes Dahlgren

PART VI. NADPH OXIDASE AND PRODUCTION OF REACTIVE OXYGEN SPECIES

23. Measurement of Respiratory Burst Products Generated by Professional Phagocytes
Claes Dahlgren, Anna Karlsson, and Johan Bylund

24. The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function
Thomas L. Leto, Mark C. Lavigne, Neda Homoyounpour, Kristen Lekstrom, Gilda Linton, Harry L. Malech, and Isabelle de Mendez

25. Cell-free Assays: The Reductionist Approach to the Study of NADPH Oxidase Assembly
Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev, Iris Dahan, Yevgeny Berdichevsky, and Edgar Pick

26. Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis

PRODUCT DETAILS

ISBN-13: 9781588297884
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2007
Pages: 564
Weight: 1004g
Availability: Available
Subcategories: Genetics, Haematology, Immunology, Physiology

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