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MORE ABOUT THIS BOOK
Main description:
A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.
Contents:
Nuclear Transplantation in Xenopus
John B. Gurdon
Nuclear Transfer in Sheep
William A. Ritchie
Protocols for Nuclear Transfer in Mice
Shaorong Gao
Isolation of Stromal Stem Cells From Human Adipose Tissue
Andrew C. Boquest, Aboulghassem Shahdadfar, Jan E. Brinchmann, and Philippe Collas
Nuclear Reprogramming by Cell Fusion
Robert H. Broyles, Austin C. Roth, Mairead Todd, and Visar Belegu
Polyethylene Glycol-Mediated Cell Fusion
Jian Yang and Ming Hong Shen
Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells
Masako Tada and Takashi Tada
Quantification of Cell Fusion by Flow Cytometry
Stephen Sullivan, Martin Waterfall, Ed J. Gallagher, Jim McWhir, and Steve Pells
Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts
Anne-Mari Hakelien, Kristine G. Gaustad, and Philippe Collas
Transgenic Systems in Nuclear Reprogramming
Megan Munsie, Peter Mountford, and Jennifer Nichols
Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos
Fatima Santos and Wendy Dean
Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies
Rong Wu, Anna V. Terry, and David M. Gilbert
Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones
Dean H. Betts, Steven Perrault, Lea Harrington, and W. Allan King
Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells
Cornelia Wiese, Gabriela Kania, Alexandra Rolletschek, Przemyslaw Blyszczuk, and Anna M. Wobus
Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens
Bruce A. Fenderson, Maria P. De Miguel, April D. Pyle, and Peter J. Donovan
Analysis of the Nucleolar Compartment of the Nucleus as anIndicator of Nuclear Reprogramming After Nuclear Transfer
Jacques-E. Flechon
Methylation-Sensitive Polymerase Chain Reaction
Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young
Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis
Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang
Chromatin Immunoprecipitation Assay for Mammalian Tissues
Fiona B. Turner, Wang L. Cheung, and Peter Cheung
Histone Modifications and Transcription Factor Binding on Chromatin: ChIP-PCR Assays
Jaejoon Won and Tae Kook Kim
In Vivo Genomic Footprinting Using LM-PCR Methods
Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer
Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease
Pascal Lefevre and Constanze Bonifer
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2010
Pages: 333
Weight: 592g
Availability: Available
Subcategories: Biochemistry
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