(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
The purpose of Ribozyme Protocols is to provide a helpful compilation of protocols that will be of use-^not only to those with some experience of ribozymes-^but also to those wishing to use ribozymes for the first time. Although it is usually impossible to cover every aspect of a scientific field, I believe this book approaches that ideal and should help all readers perform meaningful experiments using ribozymes. To design ribozymes, one must consider whether the target site will be accessible; this task can be facilitated by using computer programs that pre dict the folding of the target RNA. Such programs are detailed in Chapters 2 and 3. If the chosen target is an RNA virus that can mutate rapidly, it makes sense to consider those parts of the genome that are least likely to change during viral replication. An example of how this can be done is described in Chapter 4. Although computer analysis may be a useful starting point to select tar get sites, there seems, at the moment, to be no guarantee that any particular chosen site will be efficiently cleaved. Some workers have deliberately bypassed this problem by using libraries of ribozyme sequences and by select ing those that actually hybridize to and/or cleave the target; these methods are described in Chapters 5 and 6.
Contents:
Ribozymes: An Introduction
Helen A. James and Philip C. Turner
Computer-Aided Calculation of the Local Folding Potential of Target RNA and its Use for Ribozyme Design
Georg Sczakiel and Martin Tabler
Computational Approaches to the Identification of Ribozyme Target Sites
William James and Elizabeth Cowe
Computer Analysis of the Conservation and Uniqueness of Ribozyme-Targeted HIV Sequences
Mary Beth DeYoung and Arnold Hampel
Selection of Accessible Sites for Ribozymes on Large RNA Transcripts
Bruce L. Frank and John Goodchild
Selection of Efficient Ribozyme Cleavage Sites in Target RNAs
Andre Lieber, Mikkel Rohde, and Michael Strauss
Chemical Synthesis, Analysis, and Purification of Ribozymes
Ravi Vinayak
A Practical Method for the Production of RNA and Ribozymes
Francine E. Wincott and Nassim Usman
Preparation of Templates for Production of Ribozymes and Substrates
Rajesh K. Gaur and Guido Krupp
Enzymatic Synthesis and Characterization of Unmodified Ribozymes and Substrates
Guido Krupp
T7 Transcript Length Determination Using Enzymatic RNA Sequencing
Andrew Siwkowski
Chemical and Enzymatic Approaches to Construct Modified RNAs
Rajesh K. Gaur and Guido Krupp
Applications of Modified Transcripts
Rajesh K. Gaur, Frank Conrad, and Guido Krupp
Cloning Strategies for Catalytic Antisense RNAs
Martin Tabler and Mina Tsagris
PCR-Based Construction of Long Hammerhead Ribozymes
Martin Zillman and Gregory Robinson
Design and Production of Asymmetric Hammerhead Ribozymes
Martin Tabler and Georg Sczakiel
Minimized Hammerhead Ribozymes
Maxine J. McCall, Philip Hendry, and Trevor J. Lockett
Design of Hairpin Ribozymes for In Vitro and Cellular Applications
Qiao Yu and John M. Burke
Design of the Hairpin Ribozyme for Targeting Specific RNA Sequences
Arnold Hampel, Mary Beth DeYoung, Scott Galasinski, and Andrew Siwkowski
Design and Preparation of Sequence Specific RNase P Ribozymes
Denis Drainas and Guido Krupp
Theoretical Considerations in Measuring Reaction Parameters
Timothy S. McConnell
Experimental Approaches for Measuring Reaction Parameters
Timothy S. McConnell
Determination of Catalytic Parameters for Hairpin Ribozymes
Mary Beth DeYoung, Andrew Siwkowski, and Arnold Hampel
Characterizing Ribozyme Cleavage Reactions
Philip Hendry, Maxine J. McCall, and Trevor J. Lockett
Defining Optimum Reaction Conditions for Hammerhead Ribozymes
Philip Hendry, Maxine J. McCall, and Trevor J. Lockett
Using Fluorescence Resonance Energy Transfer to Investigate Hammerhead Ribozyme Kinetics
Thomas A. Perkins and John Goodchild
Design of Hybridizing Arms in Hammerhead Ribozymes
Philip Hendry, Maxine J. McCall, and Trevor J. Lockett
Optimization of Hammerhead Flanking Sequences Using Oligonucleotide Facilitators
John Goodchild
Enhancement of Ribozyme Function by RNA Binding Proteins
Nan Sook Lee, Edouard Bertrand, and John J. Rossi
Selection of Fast-Hybridizing Complementary RNA Species In Vitro
Ralf Kronenwett and Georg Sczakiel
In Vitro Selection of Hairpin Ribozymes
Bruno Sargueil and John M. Burke
The Detection of Hammerhead Ribozyme Cleavage by RT-PCR Methods
Rhonda Perriman
Detection of Ribozyme Cleavage Products Using Reverse Ligation Mediated PCR (RL-PCR)
Edouard Bertrand, Micheline Fromont-Racine, Raymond Pictet, and Thierry Grange
Quantitation of Ribozyme Target Abundance by QCPCR
Amber A. Beaudry and James A. McSwiggen
Trans-splicing Reactions by Ribozymes
Joshua T. Jones, Seong-Wook Lee, and Bruce A. Sull
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2013
Pages: 492
Weight: 737g
Availability: Available
Subcategories: Biochemistry
From the same series
