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Main description:
Steroid Receptors: Methods and Protocols presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Steroid Receptors: Methods and Protocols offers an updated view on a variety of modern methods that will hopefully contribute to improving our knowledge on the integration of steroid receptors in single or different functionally connected cellular contexts under a variety of physiological and pathological conditions, above all tumors.
Contents:
1. Progesterone Receptor Interaction with Chromatin
Guillermo P. Vicent, A. Silvina Nacht, Cecilia Ballare, Roser Zaurin, Daniel Soronellas, and Miguel Beato
2. Mapping the Genomic Binding Sites of the Activated Retinoid X Receptor in Murine Bone Marrow-Derived Macrophages using Chromatin Immunoprecipitation Sequencing
Bence Daniel, Balint L. Balint, Zsuzsanna S. Nagy, Laszlo L. Nagy
3. Analysis of Chromatin-Nuclear Receptor Interactions by Laser-Chromatin Immunoprecipitation
Rosaria Benedetti, Mariarosaria Conte, Vincenzo Carafa, Bartolomeo Della Ventura, Carlo Altucci, Raffaele Velotta, Hendrik G. Stunnenberg, Lucia Altucci, and Angela Nebbioso
4. Examining Estrogen Regulation of Cancer Stem Cells through Multicolor Lineage Tracing
Yang Zhang, Gabriel Eades, and Qun Zhou
5. Reporter Mice for the Study of Long Term Effects of Drugs and Toxic Compounds
Nicoletta Rizzi, Balaji Ramachandran, Cristina Vantaggiato, Paolo Ciana, and Adriana Maggi
6. Analysis of Histone post-Translational Modifications in the Control of Chromatin Plasticity Observed at Estrogen-Responsive Sites in Human Breast Cancer Cells
Annalisa Di Santi, Gustavo Cernera, Antimo Migliaccio, and Bruno Perillo
7. In Silico Analysis of Genomic Data for Construction of Nuclear Receptor Network
Yun-Young Park and Ju-Seog Lee
8. Cofactor Profiling of the Glucocorticoid Receptor from a Cellular Environment
Sofie J. Desmet, Lien Dejager, Dorien Clarisse, Jonathan Thommis, Diana Melchers, Niek Bastiaensen, Rob Ruijtenbeek, Ilse M. Beck, Claude Libert, Rene Houtman, Onno C. Meijer, and Karolien De Bosscher
9. Paxillin and Steroid Signaling: from Frog to Human
Stephen R. Hammes, Susanne U. Miedlich, and Aritro Sen
10. Analysis of the Androgen Receptor/Filamin A Complex in Stromal Cells
Pia Giovannelli, Marzia di Donato, Ferdinando Auricchio, and Gabriella Castoria
11. Multi-Well Plate Immunoassays for Measuring Signaling Protein Activations/Deactivations and Membrane Vs. Intracellular Receptor Levels
Cheryl. S. Watson, Yow-Jiun Jeng, Nataliya N. Bulayeva, Celeste C. Finnerty, Luke Y. Koong, Dragoslaca Zivadinovic, Rebecca A. Alyea, Terumi Midoro-Horiuti, Randall M. Goldblum, Noelle. C. Anastasio, Kathryn A. Cunningham, Patricia K. Seitza and Thressa D. Smith
12. Promixity Ligation Assay to Detect and Localize the Interactions of ERa with PI3-K and Src in Breast Cancer Cells and Tumor Samples
Coralie Poulard, Juliette Rambaud, Muriel Le Romancer, and Laura Corbo
13. Phosphoinositide 3-Kinase Assay in Breast Cancer Cell Extracts
Antonio Bilancio and Antimo Migliaccio
14. Rapid Estrogen Effects on Aromatase Phosphorylation in Breast Cancer Cells
Stefania Catalano, Ines Barone, and Sebastiano Ando
15. Mouse Monoclonal Antibodies against Estrogen Receptor
Caterina De Rosa, Valentina Rossi, and Ciro Abbondanza
16. Analysis of Estrogen Receptor Interacting Proteins Using Pull Down Assay and MALDI-MS Methods
Mahendra Kumar Thakur and Vijay Paramanik
17. Analysis of the Conformation of the Androgen Receptor in Spinal Bulbar Muscular Atrophy by Atomic Force Microscopy
Tobias Jochum and Andrew C. B. Cato
18. Imaging of Corticosteroid Receptors in Live Cells
Mayumi Nishi
19. Physiological Techniques in the Study of Rapid Aldosterone Effects
Yamil R. Yusef, Warren Thomas, and Brian J. Harvey
20. Detection of the Glucocorticoid Receptors in Brain Protein Extracts by SDS-PAGE
Fernanda Marques, Joao C. Sousa, Joao J. Cerqueira, and Nuno Sousa
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2014
Pages: 230
Weight: 6952g
Availability: Available
Subcategories: Biochemistry
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