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MORE ABOUT THIS BOOK
Main description:
In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.
Contents:
Part I. ELISPOT Assay Setup and Data Analysis
The ELISPOT Assay: A Personal Retrospective
Jonathon D. Sedgwick
Chemistry and Biology of the ELISPOT Assay
Alexander E. Kalyuzhny
Membranes and Membrane Plates Used in ELISPOT
Alan J. Weiss
Standardization and Validation Issues of the ELISPOT Assay
Sylvia Janetzki, Josephine H. Cox, Neal Oden, and Guido Ferrari
A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay
Angela Grant, Sarah Palzer, Chris Hartnett, Tanya Bailey, Monica Tsang, and Alexander E. Kalyuzhny
Isolation of Subsets of Immune Cells
Carrie E. Peters, Steven M. Woodside, and Allen C. Eaves
Image Analysis and Data Management of ELISPOT Assay Results
Paul Viktor Lehmann
High Resolution as a Key Feature to Perform Accurate ELISPOT Measurements Using Zeiss KS ELISPOT Readers
Wolf Malkusch
Part II. ELISPOT Assay in Animal Models
Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific Cellular Immune Responses in Rhesus Macaques
K. Jagannadha Sastry, Pramod N. Nehete, and Bharti Nehete
Feline Cytokine ELISPOT: Issues in Assay Development
Sushila K. Nordone, Rosemary Stevens, Alora S. LaVoy, and Gregg A. Dean
Measuring T-Cell Function in Animal Models of Tuberculosis by ELISPOT
Vanja Lazarevic, Santosh Pawar, and JoAnne Flynn
Interferon-g ELISPOT Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+ T-Cells
Geoffrey A. Cole
Part III. ELISPOT Assays in Human Studies
Detection of Measles Virus-Specific Interferon-g-Secreting T-Cells by ELISPOT
Jenna E. Ryan, Inna G. Ovsyannikova, and Gregory A. Poland
Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assay
Clara M. Pelfrey and Ioana R. Moldovan
Use of Interferon-g ELISPOT in Monitoring Immune Responsesin Humans
Mark Matijevic and Robert G. Urban
ELISPOT Determination of Interferon-g T-Cell Frequencies in Patients With Autoimmune Sensorineural Hearing Loss
C. Arturo Solares and Vincent K. Tuohy
Part IV. Multiplex and Modified ELISPOT Assay Formats
Dual-Color ELISPOT Assay for Analyzing Cytokine Balance
Yoshihiro Okamoto and Mikio Nishida
Simultaneous Detection of Multiple Cytokines in ELISPOT Assays
Sarah Palzer, Tanya Bailey, Chris Hartnett, Angela Grant, Monica Tsang, and Alexander E. Kalyuzhny
Fluorospot Assay: Methodological Analysis
Agnes Gazagne, Wolf Malkusch, Benoit Vingert, Wolf H. Fridman, and Eric Tartour
A Gel-Based Dual Antibody Capture and Detection Method for Assaying of Extracellular Cytokine Secretion: EliCell
Lisa A. Spencer, Rossana C. N. Melo, Sandra A. C. Perez, and Peter F. Weller
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 346
Weight: 504g
Availability: Available
Subcategories: Immunology
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