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Cytoskeleton Methods and Protocols

Series: Methods in Molecular Biology

by Ray H. Gavin

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Main description:

In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular Biology (TM) series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls.

Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research.

Contents:

Part I: Live-Cell Imaging of the Cytoskeleton

1. High Resolution Multimode Light Microscopy of Cell Migration: Long-Term Imaging and Analysis
Torsten Woellert and George M. Langford

2. Imaging the Cytoskeleton in Live Xenopus laevis Embryos
Sarah Woolner, Ann L. Miller, and William M. Bement

3. Live-Cell Imaging of the Cytoskeleton and Mitochondrial-Cytoskeletal Interactions in Budding Yeast
Theresa C. Swayne, Thomas G. Lipkin, and Liza A. Pon

Part II: Dynamics of Actin Filaments and Microtubules

4. Jasplakinolide: An Actin-Specific Reagent that Promotes Actin Polymerization
Andreas Holzinger

5. Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts
Christiane Wiese, Jonathan R. Mayers, and Alison J. Albee

Part III: Fluorescence Microscopy Protocols for Investigating Cytoskeleton Components

6. Quantitative Fluorescence Microscopy Techniques
Alessandro Esposito, Simon Schlachter, Gabriele S. Kaminski Schierle, Alan D. Elder, Alberto Diaspro, Fred S. Wouters, Clemens F. Kaminski, and Asparouh I. Iliev

7. A FRET-Based Approach for Studying Conformational Changes of a Cytoskeleton-Related Tumor Suppressor Molecule
Robert F. Hennigan, Mary F. Chaiken, Lauren A. Foster, and Wallace Ip

8. Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots
Julia Dyachok, Cheol-Min Yoo, Karuppaiah Palanichelvam, and Elison B. Blancaflor

9. Imaging of the Cytoskeleton and Mitochondria in Fixed Budding Yeast Cells
Theresa C. Swayne, Istvan R. Boldogh, and Liza A. Pon

Part IV: Electron Microscopy Protocols for Imaging the Cytoskeleton

10. Imaging Cytoskeleton Components by Electron Microscopy
Tatyana Svitkina

11.Purification and Localization of Intraflagellar Transport Particles and Polypeptides
Roger D. Sloboda

12. High Pressure Freezing, Electron Microscopy, and Immuno-Electron Microscopy of Tetrahymena thermophila Basal Bodies
Janet B. Meehl, Thomas H. Giddings, Jr., and Mark Winey

13. Strategies for Imaging Microtubules in Plant Cells
Andreas Holzinger, Eiko Kawamura, and Geoffrey O. Wasteneys

Part V: Analysis of Cell and Organelle Motility

14. A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity
Carola Luise Holweg

15. Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells
Christina King-Smith

16. Analysis of Properties of Cilia Using Tetrahymena thermophila
Vidyalakshmi Rajagopalan, Elizabeth O. Corpuz, Mark J. Hubenschmidt, Caroline R. Townsend, David J. Asai, and David E. Wilkes

17. Application of Cell Traction Force Microscopy for Cell Biology Research
James H-C. Wang and Bin Li

18. 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics
Deborah Wessels, Spencer Kuhl, and David R. Soll

19. Detergent-Extracted Models for the Study of Cilia or Flagella
Charles B. Lindemann and Kathleen A. Lesich

Part VI: Genetic and Proteomics Approaches for Cytoskeleton Protein Function

20. The Analysis of Intermediate Filament Dynamics Using Transfections and Cell Fusions
Jesus M. Paramio

21. Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila
Qi He

22. Proteomic Tools for the Analysis of Cytoskeleton Proteins
Ewa Wywial, Vishnubhat Nataraj Dongre, and Shaneen M. Singh

PRODUCT DETAILS

ISBN-13: 9781607613756
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2009
Pages: 403
Weight: 1058g
Availability: Available
Subcategories: Biochemistry, Physiology

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