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Main description:
Protein misfolding is a key feature of many disorders in humans, given that over twenty proteins are known to misfold and cause disease. In Protein Folding, Misfolding, and Disease: Methods and Protocols, experts in the field present a collection of current methods for studying the analysis of protein folding and misfolding, featuring strategies for expressing and refolding recombinant proteins which can then be utilized in subsequent experiments. This detailed volume also covers methods for analyzing the formation of amyloid, protocols for determining the size and structure of native and misfolded proteins, as well as specific examples of where misfolded proteins can be examined using state-of –the-art technologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Up to date and authoritative, Protein Folding, Misfolding, and Disease: Methods and Protocols offers researchers the tools necessary to move ahead in this vital field.
Feature:
Brings together a thorough collection of cutting-edge methods for protein folding and misfolding
Features concise, step-by-step approaches to the varying topics
Includes key tips and implementation advice from the experts
Back cover:
Protein misfolding is a key feature of many disorders in humans, given that over twenty proteins are known to misfold and cause disease. In Protein Folding, Misfolding, and Disease: Methods and Protocols, experts in the field present a collection of current methods for studying the analysis of protein folding and misfolding, featuring strategies for expressing and refolding recombinant proteins which can then be utilized in subsequent experiments. This detailed volume also covers methods for analyzing the formation of amyloid, protocols for determining the size and structure of native and misfolded proteins, as well as specific examples of where misfolded proteins can be examined using state-of –the-art technologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Up to date and authoritative, Protein Folding, Misfolding, and Disease: Methods and Protocols offers researchers the tools necessary to move ahead in this vital field.Contents:
1. Strategies for Boosting the Accumulation of Correctly Folded Recombinant Proteins Expressed in Escherichia coli
           Ario de Marco
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2. An Escherichia coli Cell-Free System for Recombinant Protein Synthesis on a Milligram Scale
           Luke A. Miles, Gabriela A.N. Crespi, Han Sen, Andrew F. Hill, and Michael W. Parker
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3. Synthesis of Peptide Sequences Derived from Fibril-Forming Proteins
           Denis B. Scanlon and John A. Karas
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4. Refolding Your Protein with a Little Help from REFOLD
           Jennifer Phan, Nasrin Yamout, Jason Schmidberger, Stephen P. Bottomley, and Ashley M. Buckle
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5. Circular Dichroism and Its Use in Protein Folding Studies
           David T. Clarke
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6. Distance Measurements by Continuous Wave EPR Spectroscopy to Monitor Protein Folding
           James A. Cooke and Louise J. Brown
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7. Solution-State Nuclear Magnetic Resonance Spectroscopy and Protein Folding
           Lisa D. Cabrita, Christopher A. Waudby, Christopher M. Dobson, and John Christodoulou
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8. Diagnostics for Amyloid Fibril Formation: Where to Begin
           Danny M. Hatters and Michael D. Griffin
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9. Probing Protein Aggregation with Quartz Crystal Microbalances
           Tuomas P.J. Knowles, Glyn L. Devlin, Christopher M. Dobson, and Mark E. Welland
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10. Dried and Hydrated X-Ray Scattering Analysis of Amyloid Fibrils
           Sally L. Gras and Adam M. Squires
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11. Solid-State NMR of Amyloid Membrane Interactions
           John D. Gehman and Frances Separovic
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12. Sedimentation Velocity Analysis of Amyloid Fibrils
           Chi Le Lan Pham, Yee-Foong Mok, and Geoffrey J. Howlett
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13. Transmission Electron Microscopy of Amyloid Fibrils
           Sally L. Gras, Lynne J. Waddington, and Kenneth N. Goldie
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14. Surface Plasmon Resonance Spectroscopy: A New Lead in Studying the Membrane Binding of Amyloidogenic Transthyretin
           Xu Hou, David H. Small, and Marie-Isabel Aguilar
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15. Elucidating the Role of Metals in Alzheimer’s Disease through the Use of Surface Enhanced Laser Desorption / Ionisation Time-of-Flight Mass Spectrometry
           Andrew D. Watt, Keyla A. Perez, and Lin Wai Hung
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: July, 2011
Pages: 230
Weight: 677g
Availability: Not available (reason unspecified)
Subcategories: Pathology
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