(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.
Contents:
Part I. Methods for Exogenous Expression of Ion Channels in Cells
Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System
Alexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk, James D. Stockand, and Qiusheng Tong
Overexpression of Proteins in Neurons Using Replication-Deficient Virus
Richard M. Ahlquist and Jane M. Sullivan
Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System
Nikita Gamper and Mark S. Shapiro
Part II. Methods for Studying Channel Structure-Function
Tertiary and Quaternary Structure Formation of Voltage-Gated Potassium Channels
John M. Robinson, Andrey Kosolapov, and Carol Deutsch
Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte Expression System
Farhad Kosari, Shaohu Sheng, and Thomas R. Kleyman
Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis
Jie Zheng
Part III. Methods for Studying Channel Regulation and Physiological Function
Probing the Effects of Phosphoinositides on Ion Channels
Chou-Long Huang
Epithelial Sodium Channel in Planar Lipid Bilayers
Bakhrom K. Berdiev and Dale J. Benos
A Simple In Vivo Method for Assessing Changes of Membrane- Bound Ion Channel Density in Xenopus Oocytes
Mouhamed S. Awayda, Weijian Shao, Ivana Vukojicic, and Abderrahmane Bengrine
Preparation of Cortical Brain Slices for Electrophysiological Recording
Costa M. Colbert
Juxtacellular Labeling and Chemical Phenotyping of Extracellularly Recorded Neurons In Vivo
Glenn M. Toney and Lynette C. Daws
Carbon Fiber Amperometry in the Study of Ion Channels and Secretion
Duk-Su Koh
Part IV. Methods for Studying Channelopathies, Genetic Screening, and Molecular Biology
Genetic Screening for Functionality of Bacterial Potassium Channel Mutants Using K+ Uptake-Deficient Escherichia coli
Lyubov V. Parfenova and Brad S. Rothberg
KCNQ1 K+ Channel-Mediated Cardiac Channelopathies
Gildas Loussouarn, Isabelle Baro, and Denis Escande
Tissue-Specific Transgenic and Knockout Mice
Andree Porret, Anne-Marie Merillat, Sabrina Guichard, Friedrich Beermann, and Edith Hummler
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: April, 2006
Pages: 228
Weight: 554g
Availability: Available
Subcategories: Biochemistry
Publisher recommends
From the same series
