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Host-Fungus Interactions

Methods and Protocols

Series: Methods in Molecular Biology

by Alexandra C. Brand and Donna M. MacCallum

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Main description:

Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease. Host-Fungus Interactions: Methods and Protocols is designed for research scientists who are involved in this work and interested in undertaking new or comparative studies of interactions between the mammalian host and clinically important fungal pathogens. Aiming to combine approaches for reverse genetics in pathogenic fungi with methods for their application in in vitro and in vivo models of disease, the book includes methods for the culture and genetic manipulation of the primary fungal pathogens and the opportunistic pathogens, as well as methods for investigating host-fungus interactions in model systems. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Host-Fungus Interactions: Methods and Protocols describes available molecular methods and fungal infection models in great detail in order to encourage researchers to try new approaches to investigating host-fungus interactions with added levels of confidence.

Contents:

Part I: Gene Disruption

1. Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy

Christoph Sasse and Joachim Morschhauser

2. Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans

Shantanu Ganguly and Aaron P. Mitchell

3. Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans

Megan D. Lenardon and Andre Nantel

4. Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi

Olga Zemska and Chad A. Rappleye

5. Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers

Min Su Kim, Seo-Young Kim, Kwang-Woo Jung, and Yong-Sun Bahn

6. Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System

Lorina G. Bakerand and Jennifer K. Lodge

7. Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast

Iran Malavazi and Gustavo Henrique Goldman

8. Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach

Fabrice N. Gravelat, David S. Askew, and Donald C. Sheppard

9. Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers

Chiung-Yu Hung, Hua Zhang Wise, and Garry T. Cole

Part II: Modulation of Gene Expression: RNAi Gene Knockdown

10. RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum

Brian H. Youseff and Chad A. Rappleye

11. RNA Interference in Cryptococcus neoformans

Michael L. Skowyra and Tamara L. Doering

12. Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA

Joao F. Menino, Agostinho J. Almeida, and Fernando Rodrigues

Part III: Modulation of Gene Expression: Regulatable Promoters

13. Tetracycline-Inducible Gene Expression in Candida albicans

Michael Weyler and Joachim Morschhauser

14. Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii

Lorina G. Baker and Jennifer K. Lodge

15. Modular Gene Over-Expression Strategies for Candida albicans

Vitor Cabral, Murielle Chauvel, Arnaud Firon, Melanie Legrand, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol Munro, and Christophe d'Enfert

Part IV: Responses to Infection In Vivo

16. Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans

Hector M. Mora-Montes, Christopher McKenzie, Judith M. Bain, Leanne E. Lewis, Lars P. Erwig, and Neil A.R. Gow

17. Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans

Joanne Gibson, Neil A.R. Gow, and Simon Y.C. Wong

18. Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils

Alieke G. Vonk, Mihai G. Netea, and Bart Jan Kullberg

19. Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions

Torsten Woellert, Christiane Rollenhagen, George M. Langford, and Paula Sundstrom

20. Simple Assays for Measuring Innate Interactions with Fungi

Ann M. Kerrigan, Maria da Gloria Teixeira de Sousa, and Gordon D. Brown

21. Binding and Uptake of Candida albicans by Human Monocyte-Derived Dendritic Cells

Annemiek B. van Spriel and Alessandra Cambi

22. Immune Responses to Candida albicans in Models of In Vitro Reconstituted Human Oral Epithelium

Jeanette Wagener, Daniela Mailander-Sanchez, and Martin Schaller

23. Analysis of Host-Cell Responses by Immunoblotting, ELISA, and Real-Time PCR

David L. Moyes and Julian R. Naglik

24. In Vitro Model of Invasive Pulmonary Aspergillosis in the Human Alveolus

Lea Gregson, William W. Hope, and Susan J. Howard

25. Biofilm Formation Studies in Microtiter Plate Format

Marta Riera, Emilia Moreno-Ruiz, Sophie Goyard, Christophe d'Enfert, and Guilhem Janbon

Part V: Responses to Infection In Vivo: Fungal Response

26. Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of

Infection

Alexandre Melo Bailao, Maristela Pereira, Silvia Maria Salem-Izacc, Clayton Luiz Borges, and Celia Maria de Almeida Soares

27. Laser Capture Microdissection of Candida albicans from Host Tissue

Caroline Westwater and David A. Schofield

28. Isolation and Amplification of Fungal RNA for Microarray Analysis from Host Samples

Anja Luttich, Sascha Brunke, and Bernhard Hube

Part VI: Responses to Infection In Vivo: Host Response

29. Cytokine Measurement Using Cytometric Bead Arrays (CBA)

Luis Castillo and Donna M. MacCallum

30. Transcript Profiling of the Murine Immune Response to Invasive Aspergillosis

Zaneeta Dhesi, Susanne Herbst, and Darius Armstrong-James

Part VII: Responses to Infection In Vivo: Model Systems for Host-Fungal Interactions

31. Caenorhabditis elegans: A Nematode Infection Model for Pathogenic Fungi

Maged Muhammed, Jeffrey J. Coleman, and Eleftherios Mylonakis

32. Drosophila melanogaster as a Model Organism for Invasive Aspergillosis

Michail S. Lionakis and Dimitrios P. Kontoyiannis

33. Galleria mellonella as a Model for Fungal Pathogenicity Testing

John Fallon, Judy Kelly, and Kevin Kavanagh

34. Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi

Ilse D. Jacobsen, Katharina Grosse, and Bernhard Hube

Part VIII: Responses to Infection In Vivo: Mammalian Hosts

35. Mouse Intravenous Challenge Models and Applications

Donna M. MacCallum

36. A Nebulized Intra-Tracheal Rat Model of Invasive Pulmonary Aspergillosis

Guillaume Desoubeaux and Jacques Chandenier

37. Invasive Models of Histoplasmosis

A. George Smulian

38. Murine Model of Concurrent Oral and Vaginal Candida albicans Colonization

Durdana Rahman, Mukesh Mistry, Selvam Thavaraj, Julian R. Naglik, and Stephen J. Challacombe

39. A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections

Donatella Pietrella, Brice Enjalbert, Ute Zeidler, Sadri Znaidi, Anna Rachini, Anna Vecchiarelli, and Christophe d'Enfert

40. Modeling of Fungal Biofilms Using a Rat Central-Vein Catheter

Jeniel E. Nett, Karen Marchillo, and David R. Andes

41. Orogastrointestinal Model of Mucosal and Disseminated Candidiasis

Karl V. Clemons and David A. Stevens

42. A Non-Lethal Murine Cutaneous Model of Invasive Aspergillosis

Ronen Ben-Ami and Dimitrios Kontoyiannis

PRODUCT DETAILS

ISBN-13: 9781617795381
Publisher: Springer (Humana Press Inc.)
Publication date: February, 2012
Pages: 613
Weight: 1339g
Availability: Available
Subcategories: General Issues, Pathology

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