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Main description:
Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols to purify and analyze the activity of the individual complement components or pathways. It includes assays that describe detection of complement SNPs, clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established "classical" assays and recently developed "new" assays to analyze the complement system activation will be useful to a wide audience of scientists.
Contents:
1. Overview
Mihaela Gadjeva
2. Evaluation of Complement Function by ELISA
Anja Roos and Jorgen Wieslander
3. Classical Complement Pathway Component C1q - Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q - Derivatives. Functional Characterization.
Mihaela Kojouharova
4. Classical Complement Pathway Components C1r and C1s - Purification from Human Serum and in Recombinant Form and Functional Characterization
Veronique Rossi, Isabelle Bally, Monique Lacroix, Gerard J Arlaud, and Nicole M Thielens
5. Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
Angela W. Norton, Paolo G.V. Martini, Lynette C. Cook , Scott Alderucci , Dianna M. Lundberg, Susan M. Fish , Charles Bedard3, John Gill, Arthur O. Tzianabos, and Michael F. Concino
6. Purification and Characterisation of human and Mouse Complement C3
Marieta M Ruseva and Meike Heurich
7. Purification of Human Complement Protein C5
Lars Sottrup-Jensen and Gregers Rom Andersen
8. Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry
Oren Moskovich and Zvi Fishelson
9. Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin
Thomas Vorup-Jensen
10. Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing
Lea Munthe-Fog, Hans Ole Madsen, and Peter Garred
11. Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System
Troels R. Kjaer and Steffen Thiel
12. Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
Misao Matsushita, David Kilpatrick, Hiroshi Shiraki, Yu Liu, Koichiro Tateishi, Mitsushi Tsujimura, Yuichi Endo, Teizo Fujita
13. Purification and Functional Analysis of Human Properdin
Joseph O`Flynn, Maria C. Faber Krol, Mohamed R. Daha, Cees van Kooten
14. Purification and Functional Characterization of C4b-binding Protein (C4BP)
Frida C. Mohlin and Anna M. Blom
15. Purification and Functional Characterization of Factor I
Sara C. Nilsson and Anna M. Blom
16. C1 inhibitor - Quantification and Purification
Lilian Varga and Jozsef Dobo
17. Purification, Quantification and Functional Analysis of Complement Factor H
Bing-Bin Yu, Beryl E. Moffatt, Marina Fedorova, Ali Ryan, Claire G.S. Villiers, James N. Arnold, Eugenie Du, Astrid Swinkels, Man Chung Li, Robert B. Sim 18. Factor H-related ProteinsMihaly Jozsi and Seppo Meri
19. Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS)
Lubka T. Roumenina, Roxane Roquigny, Caroline Blanc, Nelly Poulain, Stephanie Ngo, Marie-Agnes Dragon-Durey, and Veronique Fremeaux-Bacchi
20. Anti-Factor H Auto Antibodies Assay
Marie-Agnes Dragon-Durey, Caroline Blanc, Lubka Roumenina, Nelly Poulain Poulain, Stephanie Ngo, Pauline Bordereau, Veronique Fremeaux-Bacchi
21. Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
Najmunisa Karbani, Eswari Dodagatta-Marri, Asif S. Qaseem, Priyaa Madhukaran, Patrick Waters, Anthony G. Tsolaki, Taruna Madan, and Uday Kishore
22. Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
Eswari Dodagatta-Marri, Asif Qaseem, Najmunisa Karbani, Anthony George Tsolaki, Patrick Waters, Taruna Madan, and Uday Kishore
23. Functional Analysis of C5a Effector Responses In Vitro and In Vivo
Christian M. Karsten, Yves Laumonnier, and Joerg Koehl
24. Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry
Luke R Donius and John H Weis
25. Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry
Luke R Donius and John H Weis
26. Purification of C1q Receptors and Functional Analysis
Berhane Ghebrehiwet and Ellinor I.B. Peerschke
27. Detection of Cell Membrane-bound CD46 using Flow Cytometry
Martin Kolev and Claudia Kemper
28. Detection of CD59 using Flow Cytometry
Martin Kolev
29. Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay
Martin Kolev
30. Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry
Gitte Krogh Nielsen and Thomas Vorup-Jensen
31. Analysis of the Complement Activation in Mice
Nirmal K. Banda and Kazue Takahashi
32. Opsonophagocytic Assay
Markryan Dwyer and Mihaela Gadjeva
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2013
Pages: 350
Weight: 8928g
Availability: Available
Subcategories: Immunology
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