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Main description:
Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.
Contents:
1. Bioluminescence Imaging: Basics and Practical Limitations
Christian E. Badr
Part I: In Vitro Bioassays
2. Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells
Junji Chida and Hiroshi Kido
3. Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter
Ludovic Tricoire and Bertrand Lambolez
4. Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture
M. Hannah Degeling, M. Sarah S. Bovenberg, Marie Tannous, and Bakhos A. Tannous
Part II: Imaging Molecular and Cellular Events
5. Split Gaussia Luciferase for Imaging Ligand-Receptor Binding
Kathryn E. Luker and Gary D. Luker
6. Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell
Takahiro Suzuki and Satoshi Inouye
7. Bioluminescence Reporter Gene-Based Detection of MicroRNAs
Hae Young Ko, Young Sik Lee, and Soonhag Kim
8. Monitoring of Transcriptional Dynamics of HIF and NF B Activities
Miguel A.S. Cavadas and Alex Cheong
9. Real-Time Bioluminescent Tracking of Cellular Population Dynamics
Dan Close, Tingting Xu, Steven Ripp, and Gary Sayler
10. Fabrication of Bioluminescent Capsules and Live-Cell Imaging
Sung Bae Kim
Part III: Imaging of Disease (Pathogenesis)
11. The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice
Scott J. Goldman and Shengkan Jin
12. Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis
Hiroshi Yamashita, Dan T. Nguyen, and Euiheon Chung
13. Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
Greetje Vande Velde, Sona Kucharikova, Patrick Van Dijck, and Uwe Himmelreich
14. Bioluminescent Imaging of Bacteria During Mouse Infection
Jonathan M. Warawa and Matthew B. Lawrenz
Part IV: Therapeutic Imaging
15. Cell-Based Bioluminescence Screening Assays
Romain J. Amante and Christian E. Badr
16. Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice
Casey A. Maguire
17. Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase
Grant K. Lewandrowski, Ciara N. Magee, Marwan Mounayar, Bakhos A. Tannous, and Jamil Azzi
Part V: Emerging Technologies
18. Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay
Sjoerd van Rijn, Thomas Wurdinger, and Jonas Nilsson
19. Non-Invasive In Vivo Monitoring of Extracellular Vesicles
Charles P. Lai, Bakhos A. Tannous, and Xandra O. Breakefield
20. In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL)
Joe Dragavon, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson, Samantha Blazquez, Kelly L. Rogers, Regis Tournebize, and Spencer Shorte
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2013
Pages: 265
Weight: 6879g
Availability: Available
Subcategories: Biochemistry, Radiology
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