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Main description:
In recent years, there have been major advances in the concepts and methodologies used in the study of retinal development at both cellular and molecular levels. These advanced methodologies have allowed and will continue to allow researchers to gain new insights into the molecular mechanisms underlying retinal development. In Retinal Development: Methods and Protocols, expert researchers in the field detail many of the protocols used for a wide range of experiments. These include protocols and techniques for manipulating gene expression in vivo, tracing cell fates with modernized classic blastomere manipulation in Xenopus and with Cre-based technique in mouse and in zebrafish, retinal regeneration and stem cell-based replacement, and ERG (function) recording and non-invasive imaging. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Retinal Development: Methods and Protocols provides methodologies crucial to the success of increasingly more complex and often challenging investigations in the fields of retinal development and other biological and biomedical research.
Contents:
Part I: Manipulation of Gene Expression in vivo
1. Conditional Control of Gene Expression in the Mouse Retina
Qian Ding and Lin Gan
2. Generation Transgenic Xenopus using Restriction Enzyme-Mediated Intergration
Mohammad Haeri and Barry E. Knox
3. In vivo Functional Analysis of Transcription Factor--response Element Interaction using Transgenic Xenopus laevis
Heithem M. El-Hodiri, Yi Pan, and Lisa E. Kelly
4. Subretinal Delivery and Electroporation in Pigmented and Nonpigmented Adult Mouse Eyes
John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Johnson, Lennart Berglin, T. Michael Redmond, Jeffrey H. Boatright
5. In Ovo Eye Electroporation
Teri L. Belecky-Adams, Scott R. Hudson, and Sarika Tiwari
6. Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene Expression in Xenopus Embryos
Sally A. Moody
Also see Chapter 8.
Part II: Tracing Cell Fate
7. Testing Retina Fate Commitment in Xenopus by Blastomere Deletion, Transplantation and Explant Culture
Sally A. Moody
8. Application of Cre-loxP Recombination for Lineage Tracing of Adult Zebrafish Retinal Stem Cells
Rajesh Ramachandran, Aaron Reifler, Jin Wan, and Daniel Goldman
9. Fate Tracing of neurogenin2-expressing Cells in the Mouse Retina using CreERTM - LacZ
Wenxin Ma and Shu-Zhen Wang
Part III: In vitro Systems
10. In Vitro Explant Culture and Related Protocols for the Study of Mouse Retinal Development
Kangxin Jin and Mengqing Xiang
11. In vitro Biochemical Assays to Monitor Rhodopsin Function
Joshua Sammons and Alecia K. Gross
12. Transfection of Primary Embryonic Chicken Retinal Cells using Cationic Lipid
Yi-Wen Hsieh and Xian-Jie Yang
13. Production of High Titer RCAS Retrovirus
Run-Tao Yan and Shu-Zhen Wang
14. Chick RPE Transdifferentiation Assay for Proneural Activities
Shu-Zhen Wang and Run-Tao Yan
Part IV: Regeneration/Stem Cells
15. Studying the Generation of Regenerated Retinal Neuron from Muller glia in the Mouse Eye
Mike O. Karl and Thomas A. Reh
16. Production and Transplantation of Retinal Cells from Human and Mouse Embryonic Stem Cells
Anna La Torre, Deepak A Lamba, Anu Jayabalu, Thomas A Reh
17. Light-induced Photoreceptor Degeneration in the Retina of the Zebrafish
Scott Taylor, Jing Chen, Jing Luo and Peter Hitchcock
18. Microarray-based Gene Profiling Analysis of Muller Glia-derived Retinal Stem Cells in Light-damaged Retinas from Adult Zebrafish
Zhao Qin and Pamela A. Raymond
Also see Chapter 8.
Part V: Function/Imaging
19: Measuring Rodent Electroretinograms to Assess Retinal Function
Molly E. Clark and Timothy W. Kraft
20. Functional Imaging of Retinal Photoreceptors and Inner Neurons using Stimulus-evoked Intrinsic Optical Signals
Xin-Cheng Yao and Yi-Chao Li
Part VI: Emerging Methodologies
21. Use of Laser Capture Microdissection for Analysis of Retinal mRNA/miRNA Expression and DNA Methylation
Laszlo Hackler Jr, Tomohiro Masuda, Verity F. Oliver, Shannath L. Merbs, and Donald J.Zack
22. Revealing Looping Organization of Mammalian Photoreceptor Genes Using Chromosome Conformation Capture (3C) Assays
Guang-Hua Peng and Shiming Chen
23. Retinal Transcriptome Profiling by Directional Next Generation Sequencing Using 100 ng of Total RNA
Matthew John Brooks, Harsha Karur Rajasimha, and Anand Swaroop
24. Exome Sequencing: Capture and Sequencing of All Human Coding Regions for Disease Gene Discovery
Rinki Ratna Priya, Harsha Karur Rajasimha, Matthew Brooks, and Anand Swaroop
25. Determination of Post-translational Modifications of Photoreceptor Differentiation Factor NRL: Focus on SUMOylation
Jerome E. Roger, Jacob Nellissery and Anand Swaroop
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: June, 2012
Pages: 381
Weight: 917g
Availability: Available
Subcategories: General Issues
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