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Liposome Methods and Protocols

Series: Methods in Molecular Biology

by Subhash C. Basu and Manju Basu

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Main description:

In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Protocols is to bring together a wide range of detailed laboratory protocols covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each protocol chapter we have detailed the materials to be used, followed by a step-by-step protocol. The Notes section of each protocol is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.

Contents:

Part I. Physical Properties

Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids
Subroto Chatterjee and Dipak K. Banerjee

Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems
Massimo Masserini, Paola Palestini, Marina Pitto, Vanna Chigorno, and Sandro Sonnino

Part II. Liposome Fusion/Modulation

Peptide-Induced Fusion of Liposomes
Eve-Isabelle Pecheur and Dick Hoekstra

Liposomes: Applications in Protein-Lipid Interaction Studies,
Sujoy Ghosh and Robert Bell

Lipids in Viral Fusion
Anu Puri, Maite Paternostre, and Robert Blumenthal

Part III. Application of Liposomes

Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells
Shimon Gatt, Tama Dinur, and Arie Dagan

Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays
Manju Basu and Subhash Basu

Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis
Roger K. Bretthauer and Dennis W. Welsh

Therapeutic Uses of Antioxidant Liposomes
William L. Stone, Shyamali Mukherjee, Milton Smith, and Salil K. Das

Targeted Gene Delivery by Virosomes
Debi P. Sarkar, Komal Ramani, and Sandeep K. Tyagi

Liposomes Containing Ligands: Binding Specificity to Selectins
Sriram Neelamegham and Khushi L. Matta

Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes
P. R. Satish and A. Surolia

Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies
Reiji Kannagi

Inhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica Peptides
Takao Taki and Naoto Oku

Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent BloodCoagulation Proteins
Francis J. Castellino and Eric H. Ellison

Index

PRODUCT DETAILS

ISBN-13: 9781617372179
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 264
Weight: 454g
Availability: Available
Subcategories: Pharmacology

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