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MORE ABOUT THIS BOOK
Main description:
This detailed volume explores the most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or large scale protein production systems are considered as well as an updated view of most options available for vaccine development, including the data obtained through experimental trials which contributes to the exploration and understanding of the immune mechanisms and immune correlates relevant in protection among different animal species. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols facilitates access to well-established protocols to those beginning in this interesting and laborious field as well as providing important basic knowledge when attempting a novel vaccine design or platform.
Contents:
1. Vaccines and Vaccination for Veterinary Viral Diseases: A General Overview
Alejandro Brun
2. Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination
Natalia Barreiro-Pineiro, Rebeca Menaya-Vargas, Alberto Brandariz-Nunez; Iria Otero-Romero, Irene Lostale-Seijo, Javier Benavente, and Jose M. Martinez-Costas
3. Plant-Based Vaccine Antigen Production
Hoang Trong Phan and Udo Conrad
4. DNA Vaccines: Experiences in the Swine Model
Francesc Accensi, Fernando Rodriguez, and Paula L. Monteagudo
5. Novel Adjuvants and Immunomodulators for Veterinary Vaccines
Peter M.H. Heegaard, Yongxiang Fang, and Gregers Jungersen
6. Polymerase Mechanism-Based Method of Viral Attenuation
Cheri A. Lee, Avery August, Jamie J. Arnold, and Craig E. Cameron
7. BacMam Platform for Vaccine Antigen Delivery
Gunther M. Keil, Reiko Pollin, Claudia Muller, Katrin Giesow, and Horst Schirrmeier
8. Laboratory-Scale Production of Replication-Deficient Adenovirus Vectored Vaccines
Susan J. Morris, Alison V. Turner, Nicola Green, and George M. Warimwe
9. Generation of Recombinant Modified Vaccinia Virus Ankara Encoding VP2, NS1, and VP7 Proteins of Bluetongue Virus
Alejandro Marin-L
opez and Javier Ortego
10. Generation of Recombinant Capripoxvirus Vectors for Vaccines and Gene Knockout Function Studies
Hani Boshra, Jinxing Cao, and Shawn Babiuk
11. Recombinant Swinepox Virus for Veterinary Vaccine Development
Hong-Jie Fan and Hui-Xing Lin
12. Generation and Selection of Orf Virus (ORFV) Recombinants
Hanns-Joachim Rziha, Joerg Rohde, and Ralf Amann
13. Polycistronic Herpesvirus Amplicon Vectors for Veterinary Vaccine Development
Anita Felicitas Meier, Andrea Sara Laimbacher, and Mathias Ackermann
14. Construction and Application of Newcastle Disease Virus-Based Vector Vaccines
Paul J. Wichgers Schreur
15. Chimeric Pestivirus Experimental Vaccines
Ilona Reimann, Sandra Blome, and Martin Beer
16. Analysis of the Cellular Immune Responses to Vaccines
Nicholas Svitek, Evans L.N. Taracha, Rosemary Saya, Elias Awino, Vish Nene, and Lucilla Steinaa
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2015
Pages: 266
Weight: 7363g
Availability: Available
Subcategories: Immunology, Veterinary Medicine
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