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Adenovirus

Methods and Protocols

Series: Methods in Molecular Biology

by Miguel Chillón and Assumpció Bosch

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Main description:

Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.

Contents:

1. Biophysical Methods to Monitor Structural Aspects of the Adenovirus Infectious Cycle

Rosa Menendez-Conejero, Ana J. Perez-Berna, Gabriela N. Condezo, Alvaro Ortega-Esteban, Marta del Alamo, Pedro J. de Pablo, and Carmen San Martin

2. Proteome Analysis of Adenovirus Using Mass Spectrometry

Sara Bergstroem Lind, Konstantin A. Artemenko, and Ulf Pettersson

3. Capsid Modification Strategies for Detargeting Adenoviral Vectors

Alan L. Parker, Angela C. Bradshaw, Raul Alba, Stuart A. Nicklin, and Andrew H. Baker

4. Use of Dodecahedron "VLPs" as an Alternative to the Whole Adenovirus

Pascal Fender

5. Study of Adenovirus and CAR Axonal Transport in Primary Neurons

Charleine Zussy and Sara Salinas

6. The Use of Chromatin Immunoprecipitation (ChIP) to Study the Binding of Viral Proteins to the Adenovirus Genome In Vivo

Yueting Zheng and Patrick Hearing

7. DNA Microarray to Analyze Adenovirus-Host Interactions

Stefania Piersanti, Enrico Tagliafico, and Isabella Saggio

8. Determination of the Transforming Activities of Adenovirus Oncogenes

Thomas Speiseder, Michael Nevels, and Thomas Dobner

9. Oncolytic Adenovirus Characterization: Activity and Immune Responses

Raul Gil-Hoyos, Juan Miguel-Camacho, and Ramon Alemany

10. The Analysis of Innate Immune Response to Adenovirus Using Antibody Arrays

Nelson C. Di Paolo and Dmitry M. Shayakhmetov

11. Engineering Adenovirus Genome by Bacterial Artificial Chromosome (BAC) Technology

Zsolt Ruzsics, Frederic Lemnitzer, and Christian Thirion

12. Construction, Production, and Purification of Recombinant Adenovirus Vectors

Susana Miravet, Maria Ontiveros, Jose Piedra, Cristina Penalva, Merce Monfar, and Miguel Chillon

13. Scalable Production of Adenovirus Vectors

Ana Carina Silva, Paulo Fernandes, Marcos F.Q. Sousa, and Paula M. Alves

14. Canine Adenovirus Downstream Processing Protocol

Meritxell Puig, Jose Piedra, Susana Miravet, and Maria Mercedes Segura

15. Production of High-Capacity Adenovirus Vectors

Florian Kreppel

16. Production of Chimeric Adenovirus

Marta Miralles, Marc Garcia, Marcos Tejero, Assumpcio Bosch, and Miguel Chillon

PRODUCT DETAILS

ISBN-13: 9781627036788
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2013
Pages: 235
Weight: 6367g
Availability: Available
Subcategories: General Issues, Microbiology

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