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Biological Aging

Methods and Protocols

Series: Methods in Molecular Biology

by Trygve O. Tollefsbol

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Main description:

This book investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions.

Contents:

Contents:

Preface

Contributors

Techniques for Analysis of Biological Aging

Trygve O. Tollefsbol

Aging Cell Culture: Methods and Observations

Sharla M. O. Phipps, Joel B. Berletch, Lucy G. Andrews,

and Trygve O. Tollefsbol

Methods to Detect Biomarkers of Cellular Senescence: The Senescence Associated b-galactosidase

Koji Itahana, Judith Campisi, and Goberdhan P. Dimri

Methods of Cell Sorting of Young and Senescent Cells

Joao F. Passos and Thomas von Zglinicki

Telomere Length Analysis

Andres Canela, Peter Klatt, and Maria A. Blasco

A Method to Detect DNA Methyltransferase I Gene Transcription In Vitro in Aging Systems

Joel B. Berletch, Lucy G. Andrews, and Trygve O.

Tollefsbol

A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro

Joel B. Berletch, Sharla M. O. Phipps, Sabrina L. Walthall, Lucy G. Andrews, and Trygve O. Tollefsbol

The Chronological Life Span of Saccharomyces Cerevisiae

Paola Fabrizio and Valter D. Longo

Caloric Restriction and Life-Span Determination of Yeast Cells

Oliver Medvedik and David A. Sinclair

Methods of Nutrigenomics and Longevity Studies in Drosophila: Effects of Diets High in Sucrose, Palmitic Acid, Soy or Beef

Jiatao Ye, Xiangqin Cui, Ann Loraine, Kerry Bynum, Nam Chul Kim, Gregory White, Maria De Luca, Mark D. Garfinkel, Xiangyi Lu, and Douglas M. Ruden

Caloric Restriction Mimetics: A Novel Approach for Biogerontology

Mark A. Lane, George S. Roth, and Donald K. Ingram

Extension of Cell Life-Span Using Exogenous Telomerase

Mo K. Kang and No-Hee Park

Inducing Cellular Senescence Using Defined Genetic Elements

Hiroshi Nakagawa and Oliver G. Opitz

Methods of Induction of Cellular Senescence Using Oxidative Stresslas Hales

Nuclear Transfer Methods to Study Aging

Lin Liu and David L. Keefe

Use of Ribozymes in Cellular Aging Research

Custer C. Deocaris, Sunil C. Kaul, Kazunari Taira, and Renu Wadhwa

Methods of Evaluation of Pharmacological Drugs Effects on Aging and Life Span in Mice

Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski

Identifying Genes that Extend Life-Span Using a High Throughput Screening System

Cuiying Chen and Roland Contreras

Application of DNA Microarray Technology to Gerontological Studies

Sergey V. Anisimov

Detection and Analysis of Somatic Mutations at a LacZ Reporter Locus in Higher Organisms: Application to Mus Musculus and Drosophila Melanogaster

Ana Maria Garcia, Rita A. Busuttil, Armando Rodriguez, Carlos Cabrera, Martha Lundell, Martijn E. T. Dolle, and Jan Vijg

Subtractive Hybridization Techniques to Study Cellular Senescence

Michal B. Sternberg and Shimon Gepstein

Mapping Genetic Polymorphisms Affecting Natural Variation in Drosophila Longevity

Maria De Luca and Jeff Leips

Quantitative Trait Locus (QTL) Mapping in Aging Systems

Hui-Chen Hsu, Lu Lu, Nengjun Yi, Gary Van Zant, Robert W. Williams, and John D. Mountz

2D Gel Proteomics: An Approach to Study Age-related Differences in Protein-abundance or -Isoform Complexity in Biological Samples

Helen Kim, Shannon Eliuk, Jessy Deshane, Sreelatha Meleth, Todd Sanderson, Anita Pinner, Gloria Robinson, Landon Wilson, Marion Kirk, and Stephen Barnes

Metabolomics in the Study of Aging and Caloric Restriction

Bruce S. Kristal, Yevgeniya I. Shurubor, Rima Kaddurah- Daouk, and Wayne R. Matson

Index

PRODUCT DETAILS

ISBN-13: 9781627039062
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2014
Pages: 432
Weight: 658g
Availability: Available
Subcategories: Endocrinology, Genetics, Geriatrics

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