Wisepress Medical Bookshop

Human Retroviruses

Methods and Protocols

Series: Methods in Molecular Biology

by Elisa Vicenzi and Guido Poli

Price

Quantity

€140.79
(To see other currencies, click on price)

Hardback

Add to basket

Overview

Products Details

Customer Reviews

MORE ABOUT THIS BOOK

Main description:

Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore "assay and function of accessory genes", largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field.

Contents:

Part I: Mapping the HIV Life Cycle

1. Virion Attachment & Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand Binding Assay

Claudia Cicala and James Arthos

2. CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5

Gongpu Zhao and Peijun Zhang

3. The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating

Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero

4. The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells

Thomas Hope

5. Imaging HIV-1 Nuclear Pre-Integration Complexes

Anna Cereseto and Mauro Giacca

6. HIV-1 Reverse Transcription

Andrea Cimarelli and Jean-Luc Darlix

7. RNase H: Specificity, Mechanisms of Action, and Antiviral Target

Karin Moelling, Felix Broecker, and John E. Kerrigan

8. HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat

Laurence Colin, Eric Verdin, and Carine Van Lint

9. HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export

Alan Cochrane

10. HIV-1 Accessory Proteins: Nef

Anke Heigele, Daniel Sauter, Jan Munch, and Frank Kirchhoff

11. HIV-1 Accessory Proteins: VpR

Richard Y. Zhao and Michael I. Bukrinsky

12. HIV-1 Accessory Proteins: Vpu and Vif

Amy Andrew and Klaus Strebel

13. SIVSM/HIV-2 Vpx Proteins : Function and Uses in the Infection of Primary Myeloid Cells

Gregory Berger and Andrea Cimarelli

14. Imaging of HIV Assembly and Release

Barbara Muller and Jacomine Krijnse-Locker

Part II: Isolation, Co-Receptor Use, and Cell Tropism of HIV-1

15. HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells

Stefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti

16. Determination of HIV-1 Co-Receptor Usage

Mariangela Cavarelli and Gabriella Scarlatti

17. The Macrophage and HIV: Basic Concepts and Methodologies

Suzanne Gartner

18. HIV Infection of Dendritic Cells

Najla Nasr, Andrew Harman, Stuart Turville and Anthony L. Cunningham

19. Histocultures (Tissue Explants) in Human Retrovirology

Anush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis

Part III: In Vivo Quantification of HIV-1

20. Single Copy Quantification of HIV-1 in Clinical Samples

Ann Wiegand and Frank Maldarelli

21. Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells

Christine Rouzioux, Adeline Melard, and Veronique Avettand-Fenoel

Part IV: Biotechnological Aspects of HIV-1

22. HIV-1 Based Lentiviral Vectors

Ying Poi Liu and Ben Berkhout

23. Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone

Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang

Part V: HTLVs

24. Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques

Greta Forlani, Roberto S. Accolla, and Giovanna Tosi

25. Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR

Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni

26. Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real Time RT-PCR (qRT-PCR)

Ilaria Cavallari, Francesca Rende, and Vincenzo Ciminale

PRODUCT DETAILS

ISBN-13: 9781627036696
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2013
Pages: 325
Weight: 8342g
Availability: Available
Subcategories: General Issues, Immunology, Microbiology

Related books
From the same series

CUSTOMER REVIEWS

Average Rating