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Main description:
Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used. Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science. Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.
Contents:
Part I. Overview
1. An Overview of CE in Clinical Analysis
David S. Hage
Part II. Applications in Clinical Chemistry
2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection
Petr Tuma and Karel Stulik
3. The Selective Determination of Sulfates, Sulfonates and Phosphates in Urine by Capillary Electrophoresis - Mass Spectrometry
Svenja-Catharina Bunz, and Christian Neususs
4. Analysis of Neurotransmitter Amino Acids by CE-LIF Detection in Biological Fluids
Angelo Zinellu, Salvatore Sotgia, Luca Deiana, and Ciriaco Carru
5. Expanded Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)
Philip Britz-McKibbin
6. Development of an |On-Site Measurement System for Salivary Stress-Related Substances Based on Microchip CE
Yoshihide Tanaka and Nahoko Naruishi
7. Simultaneous Determination of Atenolol and Amiloride by CZE with Capacitively Coupled Conductivity Detection (C4D)
Khaldun M. AL Azzam and Hassan Y. Aboul-Enein
8. A Homogeneous Immunoassay of Thyroxine Based on Microchip Electrophoresis and Chemiluminescence Detection
Shulin Zhao and Yi-Ming Liu
9. High-Throughput Profiling of the Serum N-Glycome on Capillary Electrophoresis Microfluidics Systems
Dieter Vanderschaeghe, Andras Guttman, and Nico Callewaert
10. Screening of Matrix Metalloproteinase Inhibitors by Microanalysis with Fluorescence Detection
Xin Hai, Erwin Adams, and Ann Van Schepdael
Part III. Applications in Hematology
11. ABO Genotyping by Capillary Electrophoresis
James Chun-I Lee, Hsing-Mei Hsieh, Hsiao-Feng Teng, and Li-Chin Tsai
12. Separation of Hemoglobin Variants by Capillary Electrophoresis
Frederic Cotton and Beatric Gulbis
13. Newborn Screening for Hemoglobinopathies Using Capillary Electrophoresis
P.C. Giordano
Part IV. Applications in Bacteriology and Virology
14. Application of CZE in the Differentiation of Staphylococcus Aureus Strains
Boguslaw Buszewski, Katarzyna Hrynkiewicz, and Ewelina Dziubakiewicz
15. Multiplex and Quantitative Pathogen Detection with High-Resolution Capillary Electrophoresis-Based Single Strand Conformation Polymorphism
Hee Sung Hwang, Gi Won Shin, Boram Chung, Jeongkyeong Na, and Gyoo Yeol Jung
16. Application of CGE to Virus Identification
Julia A. Fruetel and Victoria A. VanderNoot
Part V. Applications in Disease Biomarker Discovery
17. Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery
Xueping Fang, Chenchen Wang, and Cheng S. Lee
18. Integrated Affinity and Electrophoresis Systems for Multiplexed Biomarker Analysis
Pamela N. Nge, Jayson V. Pagaduan, Weichun Yang, and Adam T. Woolley
19. Urinary Proteomics Based on Capillary Electrophoresis Coupled to Mass Spectrometry in Kidney Disease
Amaya Albalat, Julia Franke, Julien Gonzalez, Harald Mischak, and Petra Zurbig
20. Immunoaffinity, Capillary Electrophoresis, and Statistics for Studying Intact Alpha 1-Acid Glycoprotein Isoforms as Atherothrombosis Biomarker
Angel Puerta, Pedro J. Martin-Alvarez, Sara Ongay, Jose C. Diez-Masa and Mercedes de Frutos
Part VI. Applications in Immunology
21. Chip-Based Immunoassays
Akwasi A. Apori and Amy E. Herr
22. CE Analysis of g-Globulin Mobility and Potential Clinical Utility
Dieter Vanderschaeghe, Evi Debruyne, Hans Van Vlierberghe, Nico Callewaert, and Joris Delanghe
23. Cytokine Analysis by Immunoaffinity Capillary Electrophoresis
Mark Mendonca and Heather Kalish
Part VII. Applications in Clinical Genetic Analysis
24. Capillary Electrophoresis for the Detection of Fragile X Expanded Alleles
Rong Mao, Pinar Bayrak-Toydemir, and Elaine Lyon
25. Analysis of Microsatellite Instability by Microfluidic-Based Electrophoresis
Natalia Elfimova, Wafa Amer, and Margarete Odenthal
26. HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease
Ewa H. Lavant and Joyce Carlson
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2012
Pages: 324
Weight: 816g
Availability: Available
Subcategories: Biochemistry, Medical Diagnosis, Pathology
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