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Main description:
Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series, Listeria monocytogenes: Methods and Protocols addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation and finally, methods for control of the organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Vital and authoritative, Listeria monocytogenes: Methods and Protocols aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research.
Contents:
Part I: Detection, Quantification, and Confirmation
1. Sampling the Production/Processing Environment for Listeria
Anca Ioana Nicolau and Andrei Sorin Bolocan
2. Traditional Methods for Isolation of Listeria monocytogenes
Rui Magalhaes, Cristina Mena, Vania Ferreira, Goncalo Almeida, Joana Silva, and Paula Teixeira
3. Confirmation of Isolates of Listeria by Conventional and Real-Time PCR
David Rodriguez-Lazaro and Marta Hernandez
Part II: Characterization and Typing
4. Serotype Assignment by Sero-Agglutination, ELISA, and PCR
Lisa Gorski
5. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes
Marion Dalmasso and Kieran Jordan
6. Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes
Beatrix Stessl, Irene Ruckerl, and Martin Wagner
7. Ribotyping and Automated Ribotyping of Listeria monocytogenes
Mazin Matloob and Mansel Griffiths
8. Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes
Corinne Amar
9. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray
Edward M. Fox and Kieran Jordan
10. Analysis of Listeria monocytogenes Subproteomes
Michel Hebraud
11. The Listeria Cell Wall and Associated Carbohydrate Polymers
Marcel R. Eugster and Martin J. Loessner
12. Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria
Mathias Schmelcher and Martin J. Loessner
13. Virulence Characterization of Listeria monocytogenes
Swetha Reddy and Mark L. Lawrence
14. Internalization Assays for Listeria monocytogenes
Andreas Kuhbacher, Pascale Cossart, and Javier Pizarro-Cerda
Part III: Strain Manipulation
15. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes
Aidan Casey and Olivia McAuliffe
16. Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method
Kathrin Rychli, Caitriona M. Guinane, Karen Daly, Colin Hill, and Paul D. Cotter
17. Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes
Reha Onur Azizoglu, Driss Elhanafi, and Sophia Kathariou
18. Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR
Roberta Mazza and Rina Mazette
19. Genome Sequencing of Listeria monocytogenes
Stephan Schmitz-Esser and Martin Wagner
20. Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels
Marta Utratna and Conor P. O'Byrne
Part IV: Control Methods
21. Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers
Stavros G. Manios and Panagiotis N. Skandamis
22. Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique
Mohammed Bahey-El-Din and Cormac G.M. Gahan
23. Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments
Martin Wagner and Beatrix Stessl
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: May, 2014
Pages: 260
Weight: 7692g
Availability: Available
Subcategories: General Issues, Microbiology, Pathology
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