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Main description:
Protein Arrays: Methods and Protocols is an introduction to protein array technology and its application to the multiplexed detection of proteins. Although protein array technology has some roots in gene array technology, it can only be described as a distant relative. Unlike DNA, with its established rules of base pairing, and therefore predictable biochemical behavior, proteins are rich with diversity. Proteins can be large or small, compact or extended, basic or acidic, hydrophobic or hydrophilic, and so on. Just as importantly, their behavior is determined by the environment in which they reside, and so the composition of the buffer in which experiments are performed has a dramatic impact on the outcome of the experiment. Thus, if the goal is to simultaneously measure the expression of a large number of proteins, these variables must be addressed. Not to be deterred, scientists have created a variety of solutions to successfully detect and ch- acterize multiple proteins simultaneously. It is the intent of this volume to introduce to the reader a set of technological solutions to the diversity problem as well as to provide the reader with some examples of practical applications of these technologies.
Contents:
Protein Arrays From cDNA Expression Libraries
Hendrik Weiner, Thomas Faupel, and Konrad Bussow
Protein Expression Arrays for Proteomics
Michele Gilbert, Todd C. Edwards, and Joanna S. Albala
Generation of Protein In Situ Arrays by DiscernArray (TM) Technology
Mingyue He
Multiplexed Protein Analysis Using Spotted Antibody Microarrays
Brian B. Haab and Heping Zhou
Target-Assisted Iterative Screening of Phage Surface Display cDNA Libraries
Alexei Kurakin, Susan Wu, and Dale E. Bredesen
Design, Construction, and Use of Tissue Microarrays
Stephen M. Hewitt
A Streptavidin-Biotin-Based Microarray Platform for Immunoassays
Petra Pavlickova and Hubert Hug
Site-Specific Immobilization of Biotinylated Proteins for Protein Microarray Analysis
Rina Y. P. Lue, Grace Y. J. Chen, Qing Zhu, Marie-Laure Lesaicherre, and Shao Q. Yao
Processing of Photoaptamer Microarrays
Helen Petach, Rachel Ostroff, Chad Greef, and Gregory M. Husar
Use of a Small Molecule-Based Affinity System for the Preparation of Protein Microarrays
Karin A. Hughes
Evaluation of Antibodies and Microarray Coatings As a Prerequisite for the Generation of Optimized Antibody Microarrays
Philipp Angenendt and Joern Gloekler
Printing Functional Protein Microarrays Using Piezoelectric Capillaries
James B. Delehanty
The Use of Precision Glass Syringes and a Noncontact Microsolenoid Dispenser for the Production of High-Throughout Low-Density Arrays
Arezou Azarani
A Protein Microarray ELISA for Screening Biological Fluids
Susan M. Varnum, Ronald L. Woodbury, and Richard C. Zangar
Protein-Domain Microarrays
Alexsandra Espejo and Mark T. Bedford
SH3 Domain Protein-Binding Arrays
Sangpen Chamnongpol and Xianqiang Li
Site-Specific Peptide Immobilization Strategies for the RapidDetection of Kinase Activity on Microarrays
Mahesh Uttamchandani, Grace Y. J. Chen, Marie-Laure Lesaicherre, Shao Q. Yao
Simultaneous Monitoring of Multiple Kinase Activities by SELDI-TOF Mass Spectrometry
Vanitha Thulasiraman, Zheng Wang, Anjali Katrekar, Lee Lomas, and Tai-Tung Yip
Cytokine Protein Arrays
Ruo-Pan Huang
Fabrication and Application of G Protein-Coupled Receptor Microarrays
Ye Fang, Brian Webb, Yulong Hong, Ann Ferrie, Fang Lai, Anthony G. Frutos, and Joydeep Lahiri
ProteinChip (R) Array-Based Amyloid b Assays
Lisa E. Bradbury, James F. LeBlanc, and Diane B. McCarthy
Serum Protein-Expression Profiling Using the ProteinChip (R) Biomarker System
Kate Gilbert, Sharel Figueredo, Xiao-Ying Meng, Christine Yip, and Eric T. Fung
Protein Arrays for Serodiagnosis of Disease
Tito Bacarese-Hamilton, Andrea Ardizzoni, Julian Gray, and Andrea Crisanti
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 300
Weight: 438g
Availability: Available
Subcategories: Biochemistry
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