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Plant Virology Protocols

From Viral Sequence to Protein Function

Series: Methods in Molecular Biology

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by Peter Nagy, Gary Foster, Elisabeth Johansen and Yiguo Hong

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Main description:

Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section of Plant Virology Protocols, Second Edition presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses. The many subjects covered in this important new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-based studies. Plant Virology Protocols, Second Edition: From viral sequence to protein function is indispensable to laboratory investigators studying plant viruses.

Feature:

Offers step by step reproducible laboratory protocols written by experts in the field of plant sciences, virology, and genomics

Focus on the novel and emerging techniques used to analyze, understand, and treat plant viruses

Covers the spectrum of plant virology protocols, from general introductions to typical plant viral proteins, techniques for investigating viral acid sequences, through the emerging area of genomics and hist factor interactions

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Contents:

Section 1 General Introduction 1. Plant virus interactions –Peter Palukaitis, John Carr and James Schoelz (EJ) 2. Role of capsid proteins – John Bol (EJ) 3. Role of plant virus movement proteins – Michael Taliansky, Lesley Torrance, Natalia O. Kalinina (EJ) 4. Multiple roles of viral replication proteins in plant RNA virus replication– Peter Nagy and Judith Pogany (PN) 5. Role of silencing suppressor proteins –Jozsef Burgyan (EJ) 6. Role of vector transmission proteins – Veronique Ziegler Graff, Veronique Brault (EJ) Section 2 Viral nucleic acid sequence/function analysis 7. In vivo analysis of Viral RNA translation- William Staplin, Allen Miller (PN) 8. In vitro analysis of translational enhancer –Aurelie Rakotondrafara, Allen Miller (YH) 9. Identification of plant virus IRES – Sek-Man Wong, Dora Chin-Yen Koh, DingXiang Liu (YH) 10. Analysis of geminivirus DNA replication by 2-D gel - Keith Saunders (YH) 11. Begomoviruses: Molecular cloning and identification of replication origin - Lilian H. Florentino, Anésia A. Santos, Francisco M. Zerbini, Elizabeth P. B. Fontes (YH) 12. Analysis of viroid replication - Ricardo Flores, María-Eugenia Gas, Diego Molina, Carmen Hernández, and José-Antonio Daròs (PN) 13. Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs- Young-Chan Kim and C. Cheng Kao (PN) 14. In situ detection of plant viruses and virus-specific products - Andy Maule and Zoltan Havelda (PN) 15. Detection of siRNAs and miRNAs- Sakari Kauppinen, Zoltan Havelda (EJ) 16. Cloning of short interfering RNAs from virus infected plants - Thien X. Ho, Rachel Rusholme, Tamas Dalmay, and Hui Wang (YH) 17. Solution structure probing of RNA structures – Marc R. Fabian and K. Andrew White (PN) 18. RNA encapsidation assay - Padmanaban Annamalaiand A. L. N. Rao (GF) Section 3 Protein analysis and investigation of protein function 19. Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus- KS. Rajendran and Peter Nagy (GF) 20. Biochemical approaches for characterizing RNA-protein complexes in preparation for high resolution structure analysis- Raúl C. Gomila and Lee Gehrke (PN) 21. Probing interactions between plant virus movement proteins and nucleic acids - Tzvi Tzfira, Vitaly Citovski (PN) 22. Movement profiles, a tool for quantitative analysis of cell-to-cell movement of plant viral movement proteins - Kateryna Trutnyeva, Pia Ruggenthaler, Elisabeth Waigmann (PN) 23. Analysis of siRNA-supressor of gene silencing interactions - Lóránt Lakatos and József Burgyán (EJ) 24. Phosphorylation analysis of plant virus proteins - Kristiina M. Mäkinen and Konstantin I. Ivanov (PN) 25. Analysis of interactions between viral replicase proteins and plant intracellular membranes- Hélène Sanfaçon and Guangzhi Zhang (PN) 26. Membrane and protein dynamics in virus-infected plant cells - Michael Goodin, Romit Chakrabarty, Sharon Yelton (PN) 27. Site-directed mutagenesis of whole viral genomes - Li liu, George Lomonossoff (YH) 28. Viral protein-nucleic acid interaction: South(North)-Western blot - Huanting Liu (YH) 29. Protein-protein interactions: yeast two hybrid system - Deyin Guo, Minna-Liisa Rajamäki, and Jari Valkonen (YH) 30. NMR analysis of viral protein structures - Andrew J. Dingley, Inken Lorenzen and Joachim Grötzinger (YH) 31. Localization of viral protein in plant cells: protein tagging –Sophie Haupt, Lesley Torrance (GF) Section 4 Microscopy/GFP/Protein tagging/infections clones and other such tools 32. Construction of infectious clones for RNA viruses -

PRODUCT DETAILS

ISBN-13: 9781588298270
Publisher: Springer (Humana Press)
Publication date: March, 2008
Pages: 677
Weight: 1208g
Availability: Not available (reason unspecified)
Subcategories: Microbiology

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Average Rating

From the reviews of the second edition:

"This book describes a wide range of techniques on the molecular analyses of plant viruses and some of their interactions with their hosts. Many techniques could also be of interest to scientists studying animal and bacterial viruses. … this book should be of great use to virology researchers who take the correct approach." (Roger Hull, Microbiology Today, February, 2009)