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Nucleic Acid Aptamers

Selection, Characterization, and Application

Series: Methods in Molecular Biology

by Günter Mayer

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Main description:

This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.

Contents:

Part I: Selection

1. Selection of Aptamers for Metabolite Sensing and Construction of Optical Nanosensors

Yi Long, Franziska Pfeiffer, Gunter Mayer, Tine Daa Schroder, Veli Cengiz OEzalp, and Lars Folke Olsen

2. SELEX of Cell-Specific RNA Aptamers

Katharina Berg, Eileen Magbanua, and Ulrich Hahn

3. Developing Aptamers by Cell-Based SELEX

Silvia Catuogno, Carla Lucia Esposito, and Vittorio de Franciscis

4. DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System

Michiko Kimoto, Ken-Ichiro Matsunaga, and Ichiro Hirao

5. Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein C

Nasim Shahidi Hamedani and Jens Muller

6. Preparation of SELEX Samples for Next-Generation Sequencing

Fabian Tolle and Gunter Mayer

7. Next Generation Analysis of Deep Sequencing Data: Bringing Light into the Black Box of SELEX Experiments

Michael Blank

Part II: Characterization

8. Aptamer Binding Studies Using MicroScale Thermophoresis

Dennis Breitsprecher, Nina Schlinck, David Witte, Stefan Duhr, Philipp Baaske, and Thomas Schubert

9. Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry

Marc Vogel and Beatrix Suess

10. Applications of Aptamers in Flow and Imaging Cytometry

Isis C. Nascimento, Arthur A. Nery, Vinicius Bassaneze, Jose E. Krieger, and Henning Ulrich

11. In Vitro and In Vivo Imaging of Fluorescent Aptamers

Ioanna Theodorou, Nam Nguyen Quang, Karine Gombert, Benoit Theze, Benoit Lelandais, and Frederic Duconge

12. Crystallographic Pursuit of a Protein-RNA Aptamer Complex

John J.G. Tesmer

Part III: Application

13. Voltammetric Aptasensor Based on Magnetic Beads Assay for Detection of Human Activated Protein C

Arzum Erdem, Gulsah Congur, and Ece Eksin

14. Apta-PCR

Alessandro Pinto, Pedro Nadal Polo, Marketa Svobodova, Teresa Mairal Lerga, and Ciara K. O'Sullivan

15. Aptamer-Based Enzyme-Capture-Assay for Measurement of Plasma Thrombin Levels

Jens Muller, Tobias Becher, Gunter Mayer, and Bernd Poetzsch

16. Application of Aptamers in Histopathology

Sarah Shigdar, Li Lv, Lifen Wang, and Wei Duan

17. Aptamer Stainings for Super-Resolution Microscopy

Maria Angela Gomes de Castro, Burkhard Rammner, and Felipe Opazo

18. Synthesis and Characterization of Aptamer Targeted SNALPs for the Delivery of siRNA

Samantha E. Wilner and Matthew Levy

19. Screening of Genetic Switches Based on the Twister Ribozyme Motif

Michele Felletti, Benedikt Klauser, and Joerg S. Hartig

PRODUCT DETAILS

ISBN-13: 9781493931965
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2015
Pages: 225
Weight: 6294g
Availability: Available
Subcategories: Biochemistry

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