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Main description:
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
Contents:
Cytochrome P450 Nomenclature, 2004
David R. Nelson
Spectral Analyses of Cytochromes P450
John B. Schenkman and Ingela Jansson
Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli
Michael P. Pritchard, Lesley McLaughlin, and Thomas Friedberg
Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems
F. Peter Guengerich and Martha V. Martin
Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System
Azara Janmohamed, Prapimpuk Thaunsukon, Elizabeth A. Shephard, and Ian R. Phillips
Cytochrome P450 Reconstitution Systems
Hiroshi Yamazaki and Tsutomu Shimada
Catalytic Assays for Human Cytochrome P450: An Introduction
Thomas K. H. Chang and David J. Waxman
Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 With 7-Ethoxyresorufin as Substrate
Thomas K. H. Chang and David J. Waxman
Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation
David J. Waxman and Thomas K. H. Chang
Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes
Thomas K. H. Chang, Charles L. Crespi, and David J. Waxman
High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6a-Hydroxylation
Charles L. Crespi, Thomas K. H. Chang, and David J. Waxman
Determination of CYP2C9-Catalyzed Diclofenac 4'-Hydroxylation by High-Performance Liquid Chromatography
Charles L. Crespi, Thomas K. H. Chang, and David J. Waxman
CYP2C19-Mediated (S)-Mephenytoin 4'-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection
Charles L. Crespi, Thomas K. H. Chang, and David J. Waxman
CYP2D6-Dependent Bufuralol 1'-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquid Chromatography With Fluorescence Detection
Charles L. Crespi, Thomas K. H. Chang, and David J. Waxman
Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation
Thomas K. H. Chang, Charles L. Crespi, and David J. Waxman
Thin-Layer Chromatography Analysis of Human CYP3A-Catalyzed Testosterone 6b-Hydroxylation
David J. Waxman and Thomas K. H. Chang
Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography With Radiometric Detection
Charles L. Crespi, Thomas K. H. Chang, and David J. Waxman
An Isocratic High-Performance Liquid Chromatography Assay for CYP7A1-Catalyzed Cholesterol 7a-Hydroxylation
David J. Waxman and Thomas K. H. Chang
Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1, CYP2, and CYP3 Families
David J. Waxman and Thomas K. H. Chang
Benzydamine N-Oxygenation as a Measure of Flavin-Containing Monooxygenase Activity
Catherine K. Yeung and Allan E. Rettie
Prochiral Sulfoxidation as a Probe for Flavin-Containing Monooxygenases
Catherine K. Yeung and Allan E. Rettie
Targeting Antipeptide Antibodies Toward Cytochrome P450 Enzymes
Robert J. Edwards
The Human Cytochrome P450 Allele Nomenclature Committee Web Site: Submission Criteria, Procedures, and Objectives
Sarah C. Sim and Magnus Ingelman-Sundberg
Genotyping for Cytochrome P450 Polymorphisms
Ann K. Daly, Barry P. King, and Julian B. S. Leathart
Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing
Tamara Vanhaecke and Vera Rogiers
Isolation of Rat Hepatocytes
Peggy Papeleu, Tamara Vanhaecke, Tom Henkens, Greetje Elaut, Mathieu Vinken, Sarah Snykers, and Vera Rogiers
Rat Hepatocyte Cultures: Conventional Monolayer and Cocultures With Rat Liver Epithelial Cells
Tom Henkens,
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 380
Weight: 557g
Availability: Available
Subcategories: Biochemistry
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