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Main description:
Recent efforts to characterize genetic variation in the human genome, coupled with the rapidly developing field of genomics, have lead directly to the development of new and innovative approaches to the identification of genes contributing to complex human diseases. In Disease Gene Identification: Methods and Protocols, expert researchers in the field provide up-to-date molecular methodologies used in the process of identifying a disease gene, from the initial stage of study design to the next stage of preliminary locus identification, and ending with stages involved in target characterization and validation. As a volume in the highly successful Methods in Molecular Biology (TM) series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and detailed tips on troubleshooting and avoiding known pitfalls.
Authoritative and essential, Disease Gene Identification: Methods and Protocols seeks to aid scientists striving toward the identification and characterization of the many disease-related genes, which may someday pave the way for more accurate and improved methods of disease diagnosis as well as vital strategies for disease treatment and prevention.
Contents:
Part I: Introduction
1. Technological Issues and Experimental Design of Gene Association Studies
Johanna K. DiStefano and Darin M. Taverna
2. Statistical Issues in Gene Association Studies
Richard M. Watanabe
3. Identification of Causal Sequence Variants of Disease in the Next Generation Sequencing Era
Christopher B. Kingsley
Part II: Methods for Gene Identification
4. Microarray-Based Genome-Wide Association Studies Using Pooled DNA
Szabolcs Szelinger, John V. Pearson, and David W. Craig
5. Medium-Throughput SNP Genotyping Using Mass Spectrometry: Multiplex SNP Genotyping Using the iPLEX (R) Gold Assay
Meredith P. Millis
6. Targeted SNP Genotyping Using the TaqMan Assay
Dorit Schleinitz, Johanna K. DiStefano, and Peter Kovacs
7. Bar-Coded, Multiplexed Sequencing of Targeted DNA Regions Using the Illumina Genome Analyzer
Szabolcs Szelinger, Ahmet Kurdoglu, and David W. Craig
Part III: Functional Characterization of Susceptibility Alleles and Loci
8. Site-Directed Mutagenesis
Patricia E. Carrigan, Petek Ballar, and Sukru Tuzmen
9. Gene Expression Profiling of Tissues and Cell Lines: A Dual Color Microarray Method
Sonsoles Shack
10. Methods for microRNA Microarray Profiling
Aarati R. Ranade and Glen J. Weiss
11. Allelic Expression Profiling to Dissect Genome-Wide Association Study Signals
Jonathan D. Gruber
12. Quantitative Polymerase Chain Reaction: Quantitative PCR Using the Comparative Cq Method
Kimberly Yeatts
13. Genomic Analysis by Oligonucleotide Array Comparative Genomic Hybridization Utilizing Formalin-Fixed Paraffin Embedded Tissues
Stephanie J. Savage and Galen Hostetter
14. RNA Mapping Protocols: Northern-Blot and Amplification of cDNA Ends
Maria Lucrecia Alvarez and Mahtab Nourbakhsh
15. High Content RNA Interference Assay: Analysis of Tau Hyperphosphorylation as a Generic Paradigm
RiLee H. Robeson and Travis Dunckley
Part IV: Alternative Approaches
16. Integrative Systems Biology Approaches to Identify and Prioritize Disease and Drug Candidate Genes
Vivek Kaimal, Divya Sardana, Eric E. Bardes, Ranga Chandra Gudivada, Jing Chen, and Anil G. Jegga
17. Identification of a Common Variant Affecting Human Episodic Memory Performance Using a Pooled Genome-Wide Association Approach: A Case Study of Disease Gene Identification
Traci L. Pawlowski and Matthew J. Huentelman
18. RNAi-Based Functional Pharmacogenomics
Sukru Tuzmen, Pinar Tuzmen, Shilpi Arora, Nathalie Meurice, Spyro Mousses, and David Azorsa
19. Genetic Predisposition to -Thalassemia and Sickle Cell Anemia in Turkey: A Molecular Diagnostic Approach
A. Nazli Basak and Sukru Tuzmen
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: January, 2011
Pages: 324
Weight: 803g
Availability: Available
Subcategories: Genetics, Pathology, Physiology
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