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Mitosis

Methods and Protocols

Series: Methods in Molecular Biology

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by Andrew D. McAinsh

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Feature:

Provides an easily accessible reference volume for investigating mitosis put together by leading researchers in the field

Presents approaches in both mammalian and chicken cell systems as well as in model single-cell and multi-cellular organisms

Covers a broad range of biochemical, genetic and cell biological methods focused on dissecting specific mitotic processes

Contains up-to-date live cell imaging-based approaches to study dynamic mitotic events

Back cover:

With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.

Contents:

1. Knock-In and Knock-Out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways
Alexis R. Barr, Deborah Zyss, and Fanni Gergely

2. Functional Dissection of Mitotic Regulators through Gene Targeting in Human Somatic Cells
Eli Berdougo, Marie-Emilie Terret, and Prasad V. Jallepalli

3. RNAi in Drosophila S2 Cells As A Tool For Studying Cell Cycle Progression
Mónica Bettencourt-Dias and Gohta Goshima

4. Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast
Marco Geymonat, Adonis Spanos, and Steven Sedgwick

5. Hydrodynamic Analysis of Human Kinetochore Complexes during Mitosis
Sarah E. McClelland and Andrew D. McAinsh

6. Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells
Pier Paolo D’Avino, Vincent Archambault, Marcin R. Przewloka, Wei Zhang, Ernest D. Laue, and David M. Glover

7. Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines
Michael H. A. Schmitz and Daniel W. Gerlich

8. Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos
Eileen O’Toole and Thomas Müller-Reichert

9. Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells
António J. Pereira, Irina Matos, Mariana Lince-Faria, and Helder Maiato

10. Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells
Suzanna L. Prosser and Andrew M. Fry

11. Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin under the Native Promoter
Masamitsu Sato, Mika Toya, and Takashi Toda

12. Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression
Christiane Klebig, Alberto Toso,Satyarebala Borusu, and Patrick Meraldi

13. Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
Michael J. Emanuele and P. Todd Stukenberg

14. Live Cell Imaging of Kinetochore Capture by Microtubules in Budding Yeast
Kozo Tanaka and Tomoyuki U. Tanaka

15. The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery
Josefin Fernius and Kevin G. Hardwick

16. Measuring Proteolysis in Mitosis
Catherine Lindon and Barbara Di Fiore

17. An In Vitro Assay for Cdc20-dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast
Scott C. Schuyler and Andrew W. Murray

18. In vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg Extracts
Hiroyuki Yamano, Michelle Trickey, Margaret Grimaldi, and Yuu Kimata

19. Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation
Adam Williamson, Lingyan Jin, and Michael Rape

20. Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive ('Pseudosubstrate') Inhibitors
Matthew K. Summers and Peter K. Jackson

PRODUCT DETAILS

ISBN-13: 9781603279925
Publisher: Springer (Humana Press)
Publication date: June, 2009
Pages: 348
Weight: 1056g
Availability: Not available (reason unspecified)
Subcategories: Genetics

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CUSTOMER REVIEWS

Average Rating

From the reviews: “McAinsh … introduces mitosis as a phenomenally complicated process of the splitting of genetic material into two daughter cells that prevents genomic instability. Twenty chapters comprise this collection of laboratory protocols of up-to-date approaches and methods … used to investigate the mechanism of mitosis at the molecular level. … The volume is intended for graduate students and researchers in the field.” (SciTech Book News, March, 2010)