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Public Health Microbiology

Methods and Protocols

Series: Methods in Molecular Biology

by John F.T. Spencer, Alicia L. Ragout De Spencer and John F. T. Spencer

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Main description:

Public Health Microbiology: Methods and Protocols is focused on microorganisms that can present a hazard to human health in the course of everyday life. There are chapters dealing with organisms that are directly pathogenic to humans, including bacteria, viruses, and fungi; on organisms that produce toxins during growth in their natural habitats; on the use of bacteriocins produced by such organisms as lactobacilli and bifidobacteria; as well as several chapters on hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, and microbiological tests for sanitation equipment in food factories. Additional chapters look at the use of animals (mice) in the study of the various characteristics of milk and their relationships with lactic acid bacteria in particular. Other chapters focus on special methods for determining particular components of milk. In particular, in Parts I and II, on bacterial and viral pathogens, special attention is given to methods for PCR detection of genes with resistance to tetracycline, as well as to Salmonella enterica; for identification and typing of Campylobacter coli; for detection of the abundance of enteric viruses, hepatitis A virus, and rotaviruses in sewage, and of bacteriophages infecting the O157:H7 strain of Escherichia coli. Part III offers methods for computerized analysis and typing of fungal isolates, for isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone.

Contents:

Part I. Bacteria

Detection of Tetracycline Resistance Genes by PCR Methods
Rustam I. Aminov, Joanne C. Chee-Sanford, Natalie Garrigues, Asma Mehboob, and Roderick I. Mackie

Integron Analysis and Genetic Mapping of Antimicrobial Resistance Genes in Salmonella enterica serotype Typhimurium
Mairead Daly and Seamus Fanning

Molecular-Based Identification and Typing of Campylobacter jejuni and C. coli
Brigid Lucey, Fiona O'Halloran, and Seamus Fanning

Molecular Genotyping Methods and Computerized Analysis for the Study of Salmonella enterica
Ana Belen Vivanco, Juan Alvarez, Idoia Laconcha, Nuria Lopez-Molina, Aitor Rementeria, and Javier Garaizar

Part II. Viruses

Detection of Infectious Rotaviruses by Flow Cytometry
Albert Bosch, Rosa M. Pinto, Jaume Comas, and Francesc-Xavier Abad

Integrated Cell Culture/PCR for Detection of Enteric Viruses in Environmental Samples
Kelly A. Reynolds

Abundance in Sewage of Bacteriophages Infecting Escherichia coli O157:H7
Maite Muniesa and Juan Jofre

Molecular Genotyping of Irish Rotavirus Strains
Fiona O'Halloran and Seamus Fanning

Hepatitis A Virus: Molecular Detection and Typing
Gloria Sanchez, Cristina Villena, Albert Bosch, and Rosa M. Pinto

Part III. Fungi

Typing Fungal Isolates: Molecular Methods and Computerized Analysis
Aitor Rementeria, Ana Belen Vivanco, Ainara Cadaval, Maria Teresa Ruesga, Sonia Brena, Jose Ponton, Guillermo Quindos, and Javier Garaizar

Fungal Isolation and Enumeration in Foods
Dante Javier Bueno, Julio Oscar Silva, and Guillermo Oliver

Determination of Aflatoxins and Zearalenone in Different Culture Media
Dante Javier Bueno and Guillermo Oliver

Part IV. Other Pathogens

Intracellular Multiplication of Legionella Species and the Influence of Amoebae on TheirIntracellular Growth in Human Monocytes: Mono Mac 6 Cells and Acanthamoeba castellanii as Suitable In Vitro Models
Birgid Neumeister

Viability of Amoebae, Fungal Conidia, and Yeasts: Rapid Assessment by Flow Cytometry
Judith A. Noble-Wang, Shangtong Zhang, Daniel Price, and Donald G. Ahearn

Detection and Differentiation of Cryptosporidium Oocysts in Water by PCR-RFLP
Lihua Xiao, Altaf A. Lal, and Jianlin Jiang

Genotyping of Cryptosporidium parvum With Microsatellite Markers
Giovanni Widmer, Xiaochuan Feng, and Sultan Tanriverdi

Immunomagnetic Separation of Pathogenic Organisms From Environmental Matrices
Gary P. Yakub and Kathleen L. Stadterman-Knauer

Detection of Erysipelothrix rhusiopathiae in Clinical and Environmental Samples
Silvana G. Fidalgo and Thomas V. Riley

Interaction Between Lactic Acid Bacteria and Gastrointestinal Nematodes of Caprine Origin
Diana Draksler, Maria Cecilia Monferran, and Silvia Gonzalez

Molecular Detection of Genes Responsible for Cyanobacterial Toxin Production in the Genera Microcystis, Nodularia, and Cylindrospermopsis
Brendan P. Burns, Martin L. Saker, Michelle C. Moffitt, and Brett A. Neilan

Part V. Bacteriocins and Other Inhibitors

Purification of Antilisterial Bacteriocins
Jean-Marc Berjeaud and Yves Cenatiempo

The Hazard Analysis and Critical Control Point System in Food Safety
Anavella Gaitan Herrera

Testing Disinfectants in the Food Factory: Phenol Coefficient Method
Anavella Gaitan Herrera

Nontraditional Method of Evaluating Disinfectants: With Isolated Microorganisms From the Food Factory
Anavella Gaitan Herrera

Microbiological Analysis of Cosmetics
Anavella Gaitan Herrera

Helicobacter pylori and Food Products: A Public Health Problem
Anavella Gaitan Herrera

Microbiological Test for

PRODUCT DETAILS

ISBN-13: 9781617373718
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 568
Weight: 825g
Availability: Available
Subcategories: Infectious Diseases

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