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Main description:
Current knowledge of the mechanisms that regulate DNA repair has grown significantly over the past years with technology advances such as RNA interference, advanced proteomics and microscopy as well as high throughput screens. The third edition of DNA Repair Protocols covers various aspects of the eukaryotic response to genomic insult including recent advanced protocols as well as standard techniques used in the field of DNA repair. Both mammalian and non-mammalian model organisms are covered in the book, and many of the techniques can be applied with only minor modifications to other systems than the one described. Written in the highly successful Methods in Molecular Biology? series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, DNA Repair Protocols, Third Edition provides expert guidance for DNA repair, recombination, and replication.
Contents:
1. DNA repair: Front and Center and Not Going Away!
Vilhelm A. Bohr
Part I. Identification of Repair Genes and Generation of Repair Mutants
2. Identification of Genes Required for Damage Survival using a Cell Based RNAi Screen against the Drosophila Genome
Dashnamoorthy Ravi and Alexander James Roy Bishop
3. Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans using Genome-wide RNAi Screening
Lone Vedel Scholer, Tine Horning Moller, Steffen Norgaard, Lotte Vestergaard and Anders Olsen.
4. Establishment of the DNA Repair-defective Mutants in DT40 Cells
Masamichi Ishiai, Emi Uchida, and Minoru Takata
Part II. Assessing DNA Damage and Repair
5. Electrophoretic Mobility Shift Assays for
Protein-DNA Complexes Involved in DNA Repair
Chun Tsai, PhD, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu
6. The Comet Assay A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair
Gunter Speit and Andreas Rothfuss
7. Detection of DNA Damage by Comet Fluorescence in situ Hybridization (Comet-FISH)
W. Schloermann and M. Glei
8. Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assay
Rui Oliveira and Bjoern Johansson
9. Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells using Quantitative PCR
Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, Bennett Van Houten
Part III. Repair of Incorrect or Damaged DNA Bases
10. In vitro DNA Mismatch Repair in Human Cells
Liya Gu, Charles M. Ensor, and Guo-Min Li
11. Eukaryotic DNA Mismatch Repair in vitro
Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, Yanbin Zhang
12. Immuno-slot Blot Assay for Detection of UVR-mediated DNA Damage
Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke
13. Quantification of DNA Photoproductions in Mammalian Cell DNAUsing Radioimmunoassay
Thomas R. Berton and David L. Mitchell
14. Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-mediated PCR
Ahmad Besaratinia and Gerd P. Pfeifer
15. Construction of Plasmids Containing Site-specific DNA Interstrand Crosslinks for Biochemical and Cell Biological Studies
Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Scharer
16. Replication-coupled DNA Interstrand Crosslink Repair in Xenopus Egg Extracts
Puck Knipscheer, Markus Raschle, Orlando D. Scharer, and Johannes C. Walter
17. In Vitro Base Excision Repair using Mammalian Cell Extracts
Jason L. Parsons and Grigory L. Dianov,
18. Using Arabidopsis Cell Extracts to Monitor Repair of DNA Base Damage in vitro
Dolores Cordoba-Canero, Teresa Roldan-Arjona and Rafael R. Ariza
19. In vitro monitoring of Base Excision Repair in Saccharomyces cerevisiae
Simone Moertl and Fred Ahne
20. Mitochondrial Base Excision Repair Assays
Ricardo Gredilla and Tinna Stevnsner
Part IV. DNA Strand Breakage and Repair
21. O-Hydroxylamine-Coupled Alkaline Gel Electrophoresis Assay for the Detection and Measurement of DNA Single-Strand Breaks
April M. Luke and Jun Nakamura
22. DNA Double Stand Break Damage and Repair Assessed by Pulse-field Gel
Electrophoresis
Helen E. Bryant
23. Quantitative Amplification of Single Stranded DNA (QAOS)
Eva-Maria Holstein and David Lydall
24. Simple, Non-radioactive Measurement of Single-stranded DNA at Telomeric, Sub-telomeric and Genomic Loci in Budding Yeast
James M Dewar and David Lydall
25. Monitoring DNA Recombination Initiated by HO Endonuclease
Neal Sugawara and James E. Haber
26. Monitoring I-SceI Induced Double Strand Break Repair in DT40 Cells
David du Plessis and Julian E. Sale
27. I-SceI-based Assays to Examine Distinct Repair Outcomes of Mammalian Chromosomal Double Strand Breaks
Amanda Gunn and Jeremy M. Stark
28. Studying Repair of a Single Protein-bound Nick in vivo using the Flp-nick System
Ida Nielsen, Anni H. Andersen, and Lotte Bjergbaek
29. Measurement of Diversification in the Immunoglobulin Light Chain Gene of DT40 Cells
Julian E. Sale
30. Live cell microscopy of DNA damage response in Saccharomyces cerevisiae
Sonia Silva, Irene Gallina, Nadine Eckert-Boulet and Michael Lisby
31. Use of Gene Targeting to Study Recombination in Mammalian Cell DNA
Repair Mutants
Jennifer J. Rahn, Gerald M. Adair and Rodney S. Nairn
32. In vitro Rejoining of Double Strand Breaks in Genomic DNA
George Iliakis, Emil Mladenov and Nge Cheong
33. An In Vitro DNA Double-Strand Break Repair Assay Based on End Joining of Defined Duplex Oligonucleotides
Kamal Datta, Shubhadeep Purkayasth, Ronald D. Neumann, and Thomas A. Winters
Part V. DNA Damage Tolerance and Checkpoints
34. Assays of Bypass Replication of Genotoxic Lesions in Cell-Free Extracts
Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone
35. Quantitative Measurement of Translesion DNA Synthesis in Mammalian Cells
Omer Ziv, Noam Diamant, Sigal Shachar, Ayal Hendel and Zvi Livneh
36. Detection of PCNA Modifications in Saccharomyces cerevisiae
Adelina A. Davies and Helle D. Ulrich
37. In vitro PCNA Modification Assays
Joanne L. Parker and Helle D. Ulrich
38. Analysis of Inhibition of DNA Replication in Irradiated Cells using the SV40 Based in vitro assay of DNA Replication
George Iliakis, Emil Mladenov, Ya Wang and Hong Yan Wang
39. CHK1 Kinase Activity Assay
Hongyan Wang and Ya Wang
40. Histone H2AX Phosphorylation: A Marker for DNA Damage
Arishya Sharma Kamini Singh, and Alexandru Almasan
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2012
Pages: 648
Weight: 1407g
Availability: Available
Subcategories: Genetics
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