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Main description:
This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Poliovirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents:
1. An Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication
Philip D Minor
2. Poliovirus Laboratory Based Surveillance: An Overview
Syed Sohail Zahoor Zaidi, Humayun Asghar, Salmaan Sharif, and Muhammad Masroor Alam
3. Isolation and Characterisation of Enteroviruses from Clinical Samples
Soile Blomqvist and Merja Roivainen
4. Isolation and Characterisation of Poliovirus in Cell Culture Systems
Bruce R. Thorley and Jason A. Roberts
5. Molecular Characterization of Polio from Environmental Samples - ISSP, The Israeli Sewage Surveillance Protocol
Lester M. Shulman, Yossi Manor, Musa Hindiyeh, Danit Sofer, and Ella Mendelson
6. Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays
Glynis Dunn
7. A Transgenic Mouse Model of Poliomyelitis
Satoshi Koike and Noriyo Nagata
8. Standardized Methods for Detection of Poliovirus Antibodies
William C. Weldon, M. Steven Oberste, and Mark A. Pallansch
9. Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis,
Typing by PCR and Real-time RT-PCR
Cara C. Burns, David R. Kilpatrick, Jane C. Iber, Qi Chen, and Olen M. Kew
10. Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative
Wenbo Xu
and Yong Zhang
11. Phylogenetic Analysis of Poliovirus Sequences
Jaume Jorba
12. Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA
Erika Bujaki
13. A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses
Mael Bessaud, Isabelle Pelletier, Bruno Blondel, and Francis Delpeyroux
14. Methods to Monitor Molecular Consistency of Oral Polio Vaccine
Konstantin Chumakov
15. Methods for the Quality Control of Inactivated Poliovirus Vaccines
Thomas Wilton
16. Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays
Janny Westdijk, Larissa van der Maas, Rimko ten Have, and Gideon Kersten
17. Identification and Analysis of Antiviral Compounds against Poliovirus
Pieter Leyssen, David Franco, Aloys Tijsma, Celine Lacroix, Armando De Palma, and Johan Neyts
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: March, 2016
Pages: 320
Weight: 8123g
Availability: Available
Subcategories: Infectious Diseases, Microbiology
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