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Fluorescence in situ Hybridization (FISH)

Protocols and Applications

Series: Methods in Molecular Biology

by Joanna M. Bridger, Emanuela V. Volpi and Keith Morris

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Fluorescence in situ Hybridization (FISH)

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Main description:

Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.

Contents:

Part I: Core Techniques

1. Fluorescence in situ Hybridization (FISH), Basic Principles, and Methodology
Elisa Garimberti and Sabrina Tosi

2. Fluorescence in situ Hybridization on DNA Halo Preparations and Extended Chromatin Fibers
Lauren S. Elcock and Joanna M. Bridger

3. Detection of Nascent RNA Transcripts by Fluorescence in situ Hybridization
Jill M. Brown and Veronica J. Buckle

4. Fluorescence in situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarrays
Brenda M. Summersgill and Janet M. Shipley

5. Fluorescence in situ Hybridization (FISH) on Tissue Cryosections
Irina Solovei

6. Multiplex Fluorescence in situ Hybridization (M-FISH)
Rhona Anderson

7. Optical Mapping of Protein-DNA Complexes on Chromatin Fibers
Beth Sullivan

8. 3D-FISH on Cultured Cells Combined with Immunostaining
Irina Solovei and Marion Cremer

Part II: Technical Advancements and Novel Adaptations

9. The Comet-FISH Assay for the Analysis of DNA Damage and Repair
Graciela Spivak

10. Direct in situ Hybridization with Oligonucleotide Functionalized Quantum Dot Probes
Laurent A. Bentolila

11. LNA-FISH for Detection of microRNAs in Frozen Sections
Asli N. Silahtaroglu

12. Chromosome Orientation Fluorescence in situ Hybridization (CO-FISH) or Strand-Specific FISH
Susan M. Bailey, Eli S. Williams, Michael N. Cornforth, and Edwin H. Goodwin

13. Combinatorial Oligo FISH: Directed Labeling of Specific Genome Domains in Differentially Fixed Cell Material and Live Cells
Eberhard Schmitt, Jutta Schwarz-Finsterle, Stefan Stei, Carmen Boxler, Patrick Muller, Andriy Mokhir, Roland Kramer, Christoph Cremer, and Michael Hausmann

14. Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturation
Daniela Moralli and Zoia L. Monaco

15. CryoFISH: Fluorescence in situ Hybridization on Ultrathin Cryosections
Sheila Q. Xie, Liron-Mark Lavitas, and Ana Pombo

16. Characterization ofChromosomal Rearrangements Using Multicolor-Banding (MCB/m-band)
Thomas Liehr, Anja Weise, Sophie Hinreiner, Hasmik Mkrtchyan, Kristin Mrasek, and Nadezda Kosyakova

17. Visualizing Nucleic Acids in Living Cells by Fluorescence in vivo Hybridization
Joop Wiegant, Anneke K. Brouwer, Hans J. Tanke, and Roeland W. Dirks

Part III: Translational FISH: Applications for Human Genetics and Medicine

18. Quality Control in FISH as Part of a Laboratory's Quality Management System
Ros Hastings

19. FlashFISH: "Same Day" Prenatal Diagnosis of Common Chromosomal Aneuploidies
Sherry Ho and Mahesh A. Choolani

20. FISH for Pre-Implantation Genetic Diagnosis
Paul N. Scriven and Caroline Mackie Ogilvie

21. PNA-FISH on Human Sperm
Franck Pellestor, Cecile Monzo, and Samir Hamamah

22. POD-FISH: A New Technique for Parental Origin Determination Based on Copy Number Variation Polymorphism
Anja Weise, Madeleine Gross, Sophie Hinreiner, Vera Witthuhn, Hasmik Mkrtchyan, and Thomas Liehr

23. Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH)
Agata Kowalska, Eva Bozsaky, and Peter F. Ambros

24. ImmunoFISH on Isolated Nuclei from Paraffin-Embedded Biopsy Material
Soo-Yong Tan and Goran Mattsson

25. Fluorescence in situ Hybridization on 3D Cultures of Tumor Cells
Karen J. Meaburn

26. Simultaneous Ultrasensitive Subpopulation Staining/Hybridization in situ (SUSHI) in HIV-1 Disease Monitoring
Bruce Patterson

Part IV: Protocols for Model Organisms

27. Detection of Prokaryotic Cells with Fluorescence in situ Hybridization
Katrin Zwirglmaier

28. FISH as a Tool to Investigate Chromosome Behavior in Budding Yeast
Harry Scherthan and Josef Loidl

29. FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabrata
Edwin C. Odoemelam, Nithya Raghavan, Wannaporn Ittiprasert, Andre Miller, Joanna M. Bridger, and Matty Knight

30. Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic

PRODUCT DETAILS

ISBN-13: 9781607617884
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2010
Pages: 465
Weight: 1177g
Availability: Available
Subcategories: Biochemistry, Genetics

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