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Main description:
Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.Â
Feature:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.Â
Contents:
1. Reflecting on Confocal Microscopy: A Personal Perspective
           John White
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2. Laser Scanning Confocal Microscopy: History, Applications And Related Optical Sectioning Techniques
           Stephen W. Paddock and Kevin W. Eliceiri
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3. Confocal Microscopy on the Internet
           Kristin L. Hazelwood, Christopher S. Murphy, Zachary C. Cunningham, Matthew J. Parry-Hill, Richard L. Ludlow, Ericka B. Ramko, Anna P. Ozarowska, Adam M. Rainey and Michael W. Davidson
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4. Using Photoshop with Images Created by a Confocal System
           Jerry Sedgewick
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5. Clearing up the Signal: Spectral Imaging and Linear Unmixing in Fluorescence Microscopy
           Timo Zimmermann, Joanne Marrison, Karen Hogg, and Peter O’Toole
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6. Low Magnification Confocal Microscopy of Tumor Angiogenesis
           George McNamara, Anna Yanai, Vazgen Khankaldyyan, Walter E. Laug, Jeff Boden, Keith Webster, Yiwen Li, and Rong Wen
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7. Confocal Imaging of Butterfly Tissue
           Craig R. Brunetti
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8. Confocal Microscopy of Cardiac Myocytes
           Robert L. Price, Stephen T. Haley, Tara Bullard, Jeffrey Davis, Thomas K. Borg, and Louis Terracio
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9. Confocal Imaging of Fluorescently Labeled Proteins in the Drosophila Larval Neuromuscular Junction
           Ian P. Coyle
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10. Confocal Imaging and Three-Dimensional Visualization of Thick Autofluorescent Specimens
           Angela V. Klaus, Valerie Schawaroch, and Kevin J. Frischmann
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11. Vital Imaging of Multicellular Spheroids
           Paulo J. Oliveira, Ed Perkins, and Jon Holy
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12. Live Confocal Analysis of Mutant and Drug Treated Drosophila Embryos
           Barbara Fasulo and William Sullivan
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13. Confocal Imaging of the Microtubule Cytoskeleton in C. elegans Embryos and Germ Cells
           Kevin F. O’Connell and Andy Golden
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14. Measurement in the Confocal Microscope
           Guy Cox
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15. A Method for Quantifying Blood Flow Distribution Among The Alveoli of the Lung
           Robert L. Conhaim, Dennis M. Heisey, and Greg E. Leverson
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16. Imaging Tools for Analysis of the Ureteric Tree in the Developing Mouse Kidney
           Luise A. Cullen-McEwen, Richard J. Young, Gabriel Fricout, Dominique Jeulin, Ian S. Harper, Frank Costantini, and John F. Bertram
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17. Evaluating Confocal Microscopy System Performance
           Robert M. Zucker
PRODUCT DETAILS
Publisher: Springer (Springer New York)
Publication date: September, 2013
Pages: 370
Weight: 942g
Availability: Not available (reason unspecified)
Subcategories: Radiology
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