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Main description:
The emerging field of prenatal gene therapy is founded on scientific and technical advances in fetal medicine, molecular biology and gene therapy. This preclinical research subject aims at applying gene therapy during pregnancy for the prevention of human diseases caused by early onset congenital or gestation related conditions. In Prenatal Gene Therapy: Concepts, Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study gene therapy, fetal medicine and medical ethics. These include detailed protocols for vector production, for breeding and husbandry of the animal models, for the surgical procedures of gene delivery in large and small animals and for the methods of gene transfer analysis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Thorough and intuitive, Prenatal Gene Therapy: Concepts, Methods and Protocols seeks to aid scientists in the further study of prenatal disease and gene therapy projects beyond the scope of fetal medicine.
Contents:
1. The Concept of Prenatal Gene Therapy
Charles Coutelle, Simon Waddington
2. Candidate Diseases for Prenatal Gene Therapy
Anna L David, Simon Waddington
3. Vector Systems for Prenatal Gene Therapy: Choosing Vectors for Different Applications
Charles Coutelle, Simon Waddington
4. Vector Systems for Prenatal Gene Therapy: Principles of Adenovirus Design and Production
Raul Alba, Andrew H Baker and Stuart A Nicklin
5. Vector Systems for Prenatal Gene Therapy: Principles of Retrovirus Vector Design and Production
Steven J Howe and Anil Chandrashekran
6. Vector Systems for Prenatal Gene Therapy: Principles of Adeno-Associated Virus Vector Design and Production
Christopher J Binny, Amit C Nathwani
7. Vector Systems for Prenatal Gene Therapy: Principles of Non-viral Vector Design and Production
Suet Ping Wong, Orestis Argyros and Richard P Harbottle
8. Use of Manipulated Stem Cells for Prenatal Therapy
Jessica L. Roybal, Pablo Laje, Jesse D. Vrecenak, Alan W. Flake
9. Animal Models for Prenatal Gene Therapy: Choosing the Right Model
Vedanta Mehta, Donald Peebles, Anna L. David
10. Animal Models for Prenatal Gene Therapy: Rodent Models for Prenatal Gene Therapy
Jessica L. Roybal, Masayuki Endo, Suzanne M.K Buckley, Bronwen R. Herbert, Simon N. Waddington, Alan W. Flake
11. Animal Models for Prenatal Gene Therapy: The Sheep Model
Khalil N. Abi-Nader, Michael Boyd, Alan Flake, Vedanta Mehta, Donald Peebles, Anna L. David
12. Animal Models for Prenatal Gene Therapy: The Non-human Primate Model
Citra N Mattar, Arijit Biswas, Mahesh Choolani, Jerry KY Chan
13. Choice of Surrogate and Physiological Markers for Prenatal Gene Therapy
Juliette Delhove, Ahad A. Rahim, Tristan R. McKay, Simon N. Waddington, Suzanne M.K. Buckley
14. Monitoring for Potential Adverse Effects of Prenatal Gene Therapy: Use of Large Animal Models with Relevance to Human Application
Vedanta Mehta, Khalil N. Abi-Nader, David Carr, Jacqueline Wallace, Charles Coutelle, Simon Waddington, , Donald Peebles, Anna L. David
15. Monitoring for Potential Adverse Effects of Prenatal Gene Therapy: Mouse Models for Developmental Aberrations and Inadvertent Germ Line Transmission
Charles Coutellle, Simon Waddington, Michael Themis
16. Monitoring for Potential Adverse Effects of Prenatal Gene Therapy:
Genotoxicity Analysis in vitro and on Small Animal Models ex vivo and in vivo
Michael Themis
17. Risks, Benefits and Ethical, Legal and Societal Considerations for Translation of Prenatal Gene Therapy to Human Application
Charles Coutelle , Richard Ashcroft
1. The Concept of Prenatal Gene Therapy
Charles Coutelle, Simon Waddington
2. Candidate Diseases for Prenatal Gene Therapy
Anna L David, Simon Waddington
3. Vector Systems for Prenatal Gene Therapy: Choosing Vectors for Different Applications
Charles Coutelle, Simon Waddington
4. Vector Systems for Prenatal Gene Therapy: Principles of Adenovirus Design and Production
Raul Alba, Andrew H Baker and Stuart A Nicklin
5. Vector Systems for Prenatal Gene Therapy: Principles of Retrovirus Vector Design and Production
Steven J Howe and Anil Chandrashekran
6. Vector Systems for Prenatal Gene Therapy: Principles of Adeno-Associated Virus Vector Design and Production
7. Vector Systems for Prenatal Gene Therapy: Principles of Non-viral Vector Design and Production
Suet Ping Wong, Orestis Argyros and Richard P Harbottle
8. Use of Manipulated Stem Cells for Prenatal Therapy
Jessica L. Roybal, Pablo Laje, Jesse D. Vrecenak, Alan W. Flake
9. Animal Models for Prenatal Gene Therapy: Choosing the Right Model
Vedanta Mehta, Donald Peebles, Anna L. David
10. Animal Models for Prenatal Gene Therapy: Rodent Models for Prenatal Gene Therapy
Jessica L. Roybal, Masayuki Endo, Suzanne M.K Buckley, Bronwen R. Herbert, Simon N. Waddington, Alan W. Flake
11. Animal Models for Prenatal Gene Therapy: The Sheep Model
Khalil N. Abi-Nader, Michael Boyd, Alan Flake, Vedanta Mehta, Donald Peebles, Anna L. David
12. Animal Models for Prenatal Gene Therapy: The Non-human Primate Model
Citra N Mattar, Arijit Biswas, Mahesh Choolani, Jerry KY Chan
13. Choice of Surrogate and Physiological Markers for Prenatal Gene Therapy
Juliette Delhove, Ahad A. Rahim, Tristan R. McKay, Simon N. Waddington, Suzanne M.K. Buckley
14. Monitoring for Potential Adverse Effects of Prenatal Gene Therapy: Use of Large Animal Models with Relevance to Human Application
Vedanta Mehta, Khalil N. Abi-Nader, David Carr, Jacqueline Wallace, Charles Coutelle, Simon Waddington, , Donald Peebles, Anna L. David
15. Monitoring for Potential Adverse Effects of Prenatal Gene Therapy: Mouse Models for Developmental Aberrations and Inadvertent Germ Line Transmission
Charles Coutellle, Simon Waddington, Michael Themis
16. Monitoring for Potential Adverse Effects of Prenatal Gene Therapy:
Genotoxicity Analysis in vitro and on Small Animal Models ex vivo and in vivo
Michael Themis
17. Risks, Benefits and Ethical, Legal and Societal Considerations for Translation of Prenatal Gene Therapy to Human Application
Charles Coutelle , Richard Ashcroft
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: May, 2012
Pages: 402
Weight: 954g
Availability: Available
Subcategories: Genetics, Obstetrics and Gynaecology
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