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Main description:
The exquisite binding specificity of antibodies has made them valuable tools from the laboratory to the clinic. Since the description of the murine hybridoma technology by Koehler and Milstein in 1975, a phenomenal number of mo- clonal antibodies have been generated against a diverse array of targets. Some of these have become indispensable reagents in biomedical research, while others were developed for novel therapeutic applications. The attractiveness of an- bodies in this regard is obvious-high target specificity, adaptability to a wide range of disease states, and the potential ability to direct the host's immune s- tem for a therapeutic response. The initial excitement in finding Paul Ehrlich's "magic bullet," however, was met with widespread disappointment when it was demonstrated that murine antibodies frequently elicit the human anti-murine an- body (HAMA) response, thus rendering them ineffective and potentially unsafe in humans. Despite this setback, advances in recombinant DNA techniques over the last 15-20 years have empowered the engineering of recombinant antibodies with desired characteristics, including properties to avoid HAMA. The ability to p- duce bulk quantities of recombinant proteins from bacterial fermentation also fueled the design of numerous creative antibody constructs. To date, the United States Food and Drug Administration has approved more than 10 recombinant antibodies for human use, and hundreds more are in the development pipeline. The recent explosion in genomic and proteomic information appears ready to deliver many more disease targets amenable to antibody-based therapy.
Contents:
Part I. Antibody Sequence and Structure
Internet Resources for the Antibody Engineer
Benny K. C. Lo and Yu Wai Chen
The Kabat Database and a Bioinformatics Example
George Johnson and Tai Te Wu
IMGT, The International ImMunoGeneTics Information System (R), http://imgt.cines.fr
Marie-Paule Lefranc
Antibody Variable Regions: Toward a Unified Modeling Method
Nicholas Whitelegg and Anthony R. Rees
Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling
Stephen E. Harding, Emma Longman, Beatriz Carrasco, Alvaro Ortega, and Jose Garcia de la Torre
Part II. Antibody-Lead Generation
PCR Cloning of Human Immunoglobulin Genes
James D. Marks and Andrew Bradbury
Antibody Humanization by CDR Grafting
Benny K. C. Lo
Selection of Human Antibodies from Phage Display Libraries
James D. Marks and Andrew Bradbury
Production of Human Single-Chain Antibodies by Ribosome Display
Mingyue He, Neil Cooley, Alison Jackson, and Michael J. Taussig
Production of Human Antibodies from Transgenic Mice
C. Geoffrey Davis, Xiao-Chi Jia, Xiao Feng, and Mary Haak-Frendscho
Selection of Internalizing Antibodies for Drug Delivery
James D. Marks
Engineering Multivalent Antibody Fragments for In Vivo Targeting
Anna M. Wu
Production of Recombinant Bispecific Antibodies
Roland E. Kontermann, Tina Voelkel, and Tina Korn
Part III. Antibody Expression and Optimization
Expression and Isolation of Recombinant Antibody Fragments in E. coli
Keith A. Charlton
Expression of Recombinant Antibodies in Mammalian Cell Lines
Paul J. Yazaki and Anna M. Wu
Human Antibody Production Using Insect-Cell Expression Systems
Mary C. Guttieri and Mifang Liang
Antibody Production in Transgenic Plants
Eva Stoger, StefanSchillberg, Richard M. Twyman, Rainer Fischer, and Paul Christou
Directed Mutagenesis of Antibody Variable Domains
Kevin Brady and Benny K. C. Lo
Antibody Affinity Maturation by Chain Shuffling
James D. Marks
Antibody Affinity Maturation by Random Mutagenesis
Ikuo Fujii
Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
Syed V. S. Kashmiri, Roberto De Pascalis, and Noreen R. Gonzales
Part IV. Antibody Characterization and Novel Applications
Antibody Purification by Column Chromatography
Andrea Murray, C. Rosamund L. Graves, Kevin Brady, and Benny K. C. Lo
Affinity Measurement Using Surface Plasmon Resonance
Robert Karlsson and Anita Larsson
Kinetic Exclusion Assays to Study High Affinity Binding Interactions in Homogeneous Solutions
Robert C. Blake II and Diane A. Blake
Characterization of Antibody-Antigen Interactions by Fluorescence Spectroscopy
Sotiris Missailidis and Kevin Brady
Antibody Epitope Mapping Using Arrays of Synthetic Peptides
Ulrich Reineke
Mapping Antibody:Antigen Interactions by Mass Spectrometry and Bioinformatics
Alexandra Huhalov, Daniel I. R. Spencer, and Kerry A. Chester
Radiometal Labeling of Antibodies and Antibody Fragments for Imaging and Therapy
Ilse Novak-Hofer, Robert Waibel, Kurt Zimmermann, Roger Schibli, Jurgen Grunberg, Kerry A. Chester, Andrea Murray, Benny K. C. Lo, Alan C. Perkins, and P. August Schubiger
Production and Characterization of Anti-Cocaine Catalytic Antibodies
Paloma de Prada and Donald W. Landry
Recombinant Immunotoxins in the Treatment of Cancer
Ira Pastan, Richard Beers, and Tapan K. Bera
Antibodies in Proteomics
Andrew R. M. Bradbury, Nileena Velappan, Vittorio Verzillo, Milan Ovecka, Roberto Marzari, Daniele Sblattero, Leslie Chasteen, Robert Siegel, and Peter
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 576
Weight: 836g
Availability: Available
Subcategories: Biochemistry, Immunology
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