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Main description:
Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Likewise, our understanding of the basic biology of somatic mtDNA mutations continues to improve. In Mitochondrial DNA: Methods and Protocols, Second Edition, specialists from eight countries share their expertise, providing detailed protocols for studying many aspects of mtDNA. The volume is divided into three sections, which cover the transduction of information from mtDNA to functionally active respiratory complexes, mitochondrial reactive oxygen species (ROS) production as well as mtDNA damage and its repair, and the identification and quantification of heteroplasmic mtDNA mutations. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Mitrochondrial DNA: Methods and Protocols, Second Edition promises to aid researchers in further expanding our knowledge of this vital area of cell biology.
Contents:
Part I: Mitochondrial DNA Replication, Transcription, Translation, and Respiratory Complex Assembly
1. Biochemical Isolation of mtDNA Nucleoids from Animal Cells
Daniel F. Bogenhagen
2. Analysis of Mitochondrial DNA by Two Dimensional Agarose Gel Electrophoresis
Aurelio Reyes, Takehiro Yasukawa, Tricia J. Gulliver, and Ian J. Holt
3. Comparative Purification Strategies for Drosophilia and Human Mitochondrial DNA Replication Proteins: DNA Polymerase y and Mitochondrial Single-Stranded DNA-Binding Protein
Marcos T. Oliveira and Laurie S. Kaguni
4. Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease
Sherine S.L. Chan and William C. Copeland
5. Preparation of Human Mitochondrial Single-Stranded DNA Binding Protein
Matthew J. Longley, Leslie A. Smith, and William C. Copeland
6. Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA
Atsushi Fukuoh and Dongchon Kang
7. Inducible Expression in Human Cells, Purification and in vitro Assays for the Mitochondrial DNA Helicase Twinkle
Steffi Goffart and Hans Spelbrink
8. Purification Strategy for Recombinant Forms of the Human Mitochondrial DNA Helicase
Tawn D. Ziebarth and Laurie S. Kaguni
9. Methods for Studying Mitochondrial Transcription Termination with Isolated Components
Paolo Loguercio Polosa, Stefania Deceglie, Marina Roberti, Maria Nicola Gadaleta, and Palmiro Cantatore
10. Oxidative Phosphorylation: Synthesis of Mitochondrially-Encoded Proteins and Assembly of Individual Structural Subunits into Functional Holoenzyme Complexes
Scot C. Leary and Florin Sasarman
Part II: Mitochondrial DNA Damage and Repair
11. Reactive Oxygen Species Production by Mitochondria
Adrian J. Lambert and Martin D. Brand
12. Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach
Sam W. Chan and Junjian Z. Chen
13. Quantitative Analysis of Oxidized Guanine, 8-oxoguanine, in Mitochondrial DNA by Immunofluorescence Method
Mizuki Ohno, Sugako Oka, and Yusaku Nakabeppu
14. In vitro Measurement of DNA Base Excision Repair in Isolated Mitochondria
Melissa M. Page and Jeffrey A. Stuart
15. Targeting Repair Proteins to the Mitochondria of Mammalian Cells through Stable Transfection, Transient Transfection, Viral Transduction, and TAT-Mediated Protein Transduction
Christopher A. Koczor, Janet W. Snyder, Inna N. Shokolenko, Allison W. Dobson, Glenn L. Wilson, and Susan P. LeDoux
16. Construction and Characterization of a Cell Line Deficient in Repair of Mitochondrial, but not Nuclear, Oxidative DNA Damage
Sugako Oka, Mizuki Ohno, and Yusaku Nakabeppu
Part III: Mitochondrial DNA Mutations
17. Mitochondrial DNA Oxidative Damage and Mutagenesis in Saccharomyces cerevisiae
Lyra M. Griffiths, Nicole A. Doudican, Gerald S. Shadel, and Paul W. Doetsch
18. Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysis
Kok Seong Lim, Robert K. Naviaux, and Richard H. Haas
19. Rapid Identification of Unknown Heteroplasmic Mitochondrial DNA Mutations with Mismatch-Specific Surveyor Nuclease
Sylvie Bannwarth, Vincent Procaccio, and Veronique Paquis-Flucklinger
20. Collection of Isolated Cells for Studying Mitochondrial DNA Mutations within Individual Cells
Yevgenya Kraytsberg, Natalya Bodyak, Susan Myerow, Alexander Nicholas, Konstantin Ebralidze, and Konstantin Khrapko
21. Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR
Yevgenya Kraytsberg, Natalya Bodyak, Susan Myerow, Alexander Nicholas, Konstantin Ebralidze, and Konstantin
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2011
Pages: 404
Weight: 874g
Availability: Available
Subcategories: Genetics
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