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Main description:
In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.
Contents:
1. General Overview of Neuronal Cell Culture
Jennifer Gordon, Shohreh Amini, and Martyn K. White
2. Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology
Jane Kovalevich and Dianne Langford
3. Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12
Nune Darbinian
4. Murine Teratocarcinoma-Derived Neuronal Cultures
Prasun K. Datta
5. Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons
Armine Darbinyan, Rafal Kaminski, Martyn K. White, Nune Darbinian, and Kamel Khalili
6. Mouse Enteric Neuronal Cell Culture
Yonggang Zhang and Wenhui Hu
7. Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications
Joseph F. Bonner, Christopher J. Haas, and Itzhak Fischer
8. Derivation of Neuronal Cells from Fetal Normal Human Astrocyte (NHA)
S. Ausim Azizi and Barbara Krynska
9. Slice Culture Modeling of Central Nervous System (CNS) Viral Infection
Kalen R. Dionne and Kenneth L. Tyler
10. Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping
Amanda Parker Struckhoff and Luis Del Valle
11. Transfection of Neuronal Cultures
Ilker Kudret Sariyer
12. Lentiviral Transduction of Neuronal Cells
Hassen S. Wollebo, Baheru Woldemichaele, and Martyn K. White
13. Compartmentalized Neuronal Cultures
Armine Darbinyan, Paul Pozniak, Nune Darbinian, Martyn K. White, and Kamel Khalili
14. Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates
George M. Smith, Yingpeng Liu, and Jee W. Hong
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2013
Pages: 175
Weight: 4831g
Availability: Available
Subcategories: Neurology, Neuroscience
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