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MORE ABOUT THIS BOOK
Main description:
This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.
Contents:
Part I In Vitro Chemical Genetic Screening
1. Identification of Therapeutic Small Molecule Leads in Cultured Cells Using Multiplexed Pathway Reporter Read-outs
Ozlem Kulak, Kiyoshi Yamaguchi, and Lawrence Lum
2. Applying the Logic of Genetic Interaction to Discover Small Molecules That Functionally Interact with Human Disease Alleles
Ari D. Brettman, Pauline H. Tan, Khoa Tran, and Stanley Y. Shaw
3. Construction and Application of a Photo-cross-linked Chemical Array
Yasumitsu Kondoh, Kaori Honda, and Hiroyuki Osada
4. High Content Screening for Modulators of Cardiac Differentiation in Human Pluripotent Stem Cells
Sean Spiering, Herman Davidovics, Paul J. Bushway, Mark Mercola, and Erik Willems
5. Small Molecule High-Throughput Screening Utilizing Xenopus Egg Extract
Matthew R. Broadus, P. Renee Yew, Stephen R. Hann, and Ethan Lee
Part II In Vivo Chemical Genetic Screening
6. Fission Yeast-based High-throughput Screens for PKA Pathway Inhibitors and Activators
Ana Santos de Medeiros, Grace Kwak, Jordan Vanderhooft, Sam Rivera, Rachel Gottlieb, and Charles S. Hoffman
7. A Method for High-throughput Analysis of Chronological Aging in Schizosaccharomyces pombe
Jessica Stephan and Ann E. Ehrenhofer-Murray
8. Protocols for the Routine Screening of Drug Sensitivity in the Human Parasite Trichomonas vaginalis
Manal J. Natto, Anthonius A. Eze, and Harry P. de Koning
9. Chemical Genetic Screens Using Arabidopsis thaliana Seedlings Grown on Solid Medium
Thanh Theresa Dinh and Xuemei Chen
10. Small Molecule Screening Using Drosophila Models of Human Neurological Disorders
Mickael Poidevin, Feiran Zhang, and Peng Jin
11. High-throughput Small Molecule Screening in Caenorhabditis elegans
Sunitha Rangaraju, Gregory M. Solis, and Michael Petrascheck
12. Whole-organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish
Philipp Gut and Didier Y.R. Stainier
13. High Content Screening for Modulators of Cardiovascular or Global Developmental Pathways in ZebrafishCharles H. Williams III and Charles C. Hong
Part III Small Molecules in Chemical Biology
14. Extraction Methods of Natural Products from Traditional Chinese Medicines
Jingyu Zhang, Jianying Han, Ayokunmi Oyeleye, Miaomiao Liu, Xueting Liu, and Lixin Zhang
15. Bioassay-guided Identification of Bioactive Molecules from Traditional Chinese Medicines
Jianying Han, Jingyu Zhang, Wenni He, Pei Huang, Ayokunmi Oyeleye, Xueting Liu, and Lixin Zhang
16. NMR Screening in Fragment-Based Drug Design: A Practical Guide
Hai-Young Kim and Daniel F. Wyss
17. Practical Strategies for Small Molecule Probe Development in Chemical Biology
Jonathan E. Hempel and Charles C. Hong
18. Principal Component Analysis as a Tool for Library Design: A Case Study Investigating Natural Products, Brand-name Drugs, Natural Product-like Libraries, and Drug-like Libraries
Todd A. Wenderski, Christopher F. Stratton, Renato A. Bauer, Felix Kopp, and Derek S. Tan
19. Small Molecule Library Screening by Docking with PyRx
Sargis Dallakyan and Arthur J. Olson
Part IV Small Molecule Target Identification
20. Fluorous Photoaffinity Labeling to Probe Protein-Small Molecule Interactions
Weigang Huang and Qisheng Zhang
21. Identification of the Targets of Biologically Active Small Molecules Using Quantitative Proteomics
Gloria Vendrell-Navarro, Andreas Brockmeyer, Herbert Waldmann, Petra Janning, and Slava Ziegler
22. Drug Affinity Responsive Target Stability (DARTS) for Small Molecule Target Identification
Melody Y. Pai, Brett Lomenick, Heejun Hwang, Robert Schiestl, William McBride, Joseph A. Loo, and Jing Huang
23. Chemical Genomic Profiling via Barcode Sequencing to Predict Compound Mode of ActionJeff S. Piotrowski, Scott W. Simpkins, Sheena C. Li, Raamesh Deshpande, Sean J. McIlwain, Irene M. Ong, Chad L. Myers, Charlie Boone, and Raymond J. Andersen
24. Image-Based Prediction of Drug Target in Yeast
Shinsuke Ohnuki, Hiroki Okada, and Yoshikazu Ohya
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: January, 2015
Pages: 275
Weight: 1125g
Availability: Available
Subcategories: Biochemistry
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