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Main description:
The aim of MHC Protocols is to document protocols that can be used for the analysis of genetic variation within the human major histocompatibility complex (MHC; HLA region). The human MHC encompasses approximately 4 million base pairs on the short arm of chromosome 6 at cytogenetic location 6p21. 3. The region is divided into three subregions. The telomeric class I region contains the genes that encode the HLA class I molecules HLA-A, -B, and -C. The centromeric class II region contains the genes encoding the HLA class II molecules HLA-DR, -DQ, and -DP. In between is the class III region, originally identified because it contains genes encoding components of the complement pathway. The entire human MHC has recently been sequenced (1) and each subregion is now known to contain many other genes, a number of which have immunological functions. The study of polymorphism within the MHC is well established, because the region contains the highly polymorphic HLA genes. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. As a result, large numbers of HLA alleles have been identified, a process that has been further driven by recent interest in HLA gene diversity in ethnic populations. The extreme genetic variation in HLA genes is believed to have been driven by the evolutionary response to infectious agents, but relatively few studies have analyzed associations between HLA genetic variation and infectious disease, which has been difficult to demonstrate.
Contents:
I. Databases
HLA Informatics: Accessing HLA Sequences from Sequence Databases
James Robinson and Steven G. E. Marsh
Accessing HLA Sequencing Data Through the 6ace Database
Roger Horton and Stephan Beck
II. Polymorphism in Classical and Nonclassical HLA Genes
HLA Typing by Restriction Fragment Length Polymorphism Analysis
Robert W. Vaughan
PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles
Robert W. Vaughan
PCR-Sequence-Specific Oliogonucleotide Probe Typing for HLA-A, -B, and -DR
Derek Middleton and F. Williams
HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes
Lori L. Steiner, Priscilla V. Moonsamy, Teodorica L. Bugawan, and Ann B. Begovich
PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles
Mike Bunce
HLA Typing With Reference Strand-Mediated Conformation Analysis
J. Rafael Arguello, Martha Perez-Rodriguez, Andrea Pay, Gaby Fisher, Alasdair McWhinnie, and J. Alejandro Madrigal
Sequencing Protocols for Detection of HLA Class I Polymorphism
Paul P. J. Dunn, Steven T. Cox, and Ann-Margaret Little
HLA-E and HLA-G Typing
Jorge Martinez-Laso, Eduardo Gomez-Casado, and Antonio Arnaiz-Villena
Typing Alleles of HLA-DM
Helene Teisserenc
III. Polymorphism in Non-HLA MHC Genes
Typing Alleles of TAP1 and TAP2
Stephen H. Powis
Determining Alleles of the C2 Gene by Southern Blotting
Zeng-Bian Zhu and John E. Volanakis
Complement C4 Protein and DNA Typing Methods
Peter M. Schneider and Gottfried Mauff
Typing of Tumor Necrosis Factor Alleles
Anthony Gerard Wilson
Molecular Typing of the MHC Class I Chain-Related Gene Locus
R. W. M. Collins, Henry A. F. Stephens, and Robert W. Vaughan
IV.Microsatellites
HLA Microsatellite Analysis
Mary Carrington
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2013
Pages: 340
Weight: 521g
Availability: Available
Subcategories: Immunology
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