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Main description:
Ovarian cancer is the most lethal malignancy of the female reproductive system and is also the fifth leading cause of cancer death in women. Sharing common characteristics of cancer, ovarian malignancy possess several clinical and biological particularities In Ovarian Cancer: Methods and Protocols, expert researchers in the field provide methods that have been created or adapted to study various aspects of ovarian cancer. These methods and techniques are applicable to study genetic alterations present in ovarian cancer, structural and metabolic features of ovarian cancer cells, include in vitro and in vivo models that recapitulate ovarian cancer development and progression, and describe ovarian cancer-oriented drug delivery approaches. Methodological chapters are grouped into seven thematic parts, any of them is introduced by short subject review. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Ovarian Cancer: Methods and Protocols seeks to aid scientist to optimize study designs, to correctly select the most applicable methods, and to produce interesting and novel results.
Contents:
Part I: Evaluation of Genomic Alteration
1. Ovarian Cancer Genome
Evgeny N. Imyanitov
2. Identifying Associations Between Genomic Alterations in Tumours
Joshy George, Kylie L Gorringe, Gordon K. Smyth, and David DL Bowtell
3. Analysis of Genome-wide DNA Methylation Profiles by BeadChip Technology
Qiong Lin, Wolfgang Wagner, and Martin Zenke
4. Integrative Prediction of Gene Function and Platinum Free Survival from Genomic and Epigenetic Features in Ovarian Cancer
Kazimierz O. Wrzeszczynski, Vinay Varadan, Sitharthan Kamalakaran, Douglas A. Levine, Nevenka Dimitrova, and Robert Lucito
5. Survival Prediction Based on Inherited Gene Variation Analysis
Mine S. Cicek, Matthew J. Maurer, and Ellen L. Goode
Part II: Targeted Analysis of DNA Methylation
6. Main Principles and Outcomes of DNA Methylation Analysis
Susan K. Murphy, Christopher F. Bassil, and Zhiqing Huang
7. Methylation-specific PCR
Zhiqing Huang, Christopher F. Bassil, and Susan K. Murphy
8. Bisulfite Sequencing of Cloned Alleles
Zhiqing Huang, Christopher F. Bassil, and Susan K. Murphy
9. Bisulfite Pyrosequencing
Christopher F. Bassil, Zhiqing Huang, and Susan K. Murphy
Part III: Gene/Genome Expression Assessing
10. RNA Networks in Ovarian Cancer
Anastasia Malek
11. Microarray-based Transcriptome Profiling of Ovarian Cancer Cells
Juan Cui, Ying Xu and David Puett
12. Deep Transcriptome Profiling of Ovarian Cancer Cells using Next Generation Sequencing Approach
Lisha Li, Jie Liu, Wei Yu, Xiaoyan Lou, Bingding Huang, and Biaoyang Lin
13. Assessment of mRNA Splice Variants by qRT-PCR
Ileabett M. Echevarria Vargas and Pablo E. Vivas-Mejia
14. Micro-RNA Profiling in Ovarian Cancer
Marilena V. Iorio and Carlo M. Croce
15. Detailed Analysis of Promoter-associated RNA
Sara Napoli
16. Integrating Multiple Types of Data to Identify MicroRNA-gene Co-modules
Shihua Zhang
Part IV: Cellular Structure and Metabolism Analysis
17. Energy Metabolism and Changes in Cellular Composition in Ovarian Cancer
Anastasia Malek
18. Metabolomic Profiling of Ovarian Carcinomas using Mass Spectrometry
Miranda Y. Fong, Jonathan McDunn, and Sham S. Kakar
19. Choline Metabolic Profiling by Magnetic Resonance Spectroscopy
Egidio Iorio, Alessandro Ricci, Maria Elena Pisanu, Marina Bagnoli, Franca Podo, and Silvana Canevari
20. Proteomic Profiling of Ovarian Cancer Models using TMT-LC-MS/MS
John Sinclair and John F. Timms
21. Characterization of Signalling Pathways by Reverse Phase Protein Arrays
Katharina Malinowsky, Claudia Wolff, Christina Schot,t and Karl-Friedrich Becker
22. N-Glycosylation Analysis by HPAEC-PAD and Mass Spectrometry
Sebastian Kandzia and Julia Costa
Part V: In Vitro Assays in Ovarian Cancer Research
23. In vivo and in vitro Properties of Ovarian Cancer Cells
Anastasia Malek
24. Establishement of Primary Cultures from Ovarian Tumour Tissue and Ascites Fluid
Brigitte L. Theriault, Lise Portelance, Anne-Marie Mes-Masson, and Mark W. Nachtigal
25. Ovarian Cancer Stem Cells Enrichment
Lijuan Yang and Dongmei Lai
26. Assessment of Resistance to Anoikis in Ovarian Cancer
Xiaoping He, Jeremy Chien, and Viji Shridhar
27. Analysis of EMT by Flow Cytometry and Immunohistochemistry
Robert Strauss, Jiri Bartek, and Andre Lieber
Part IV: Models of Ovarian Carcinogenesis
28. Challenges in Experimental Modeling of Ovarian Cancerogenesis
Jim Petrik
29. Transformation of the Human Ovarian Surface Epithelium with Genetically-defined Elements
Weiwei Shan and Jinsong Liu
30. In vitro Model of Spontaneous Mouse OSE Transformation
Paul C. Roberts and Eva M. Schmelz
31. Orthotopic, Syngeneic Mouse Model to Study the Effects of Epithelial-Stromal Interaction
James B. Greenaway and Jim J. Petrik
32. Immunocompetent Mouse Model of Ovarian Cancer for in vivo Imaging
Selene Nunez-Cruz and Nathalie Scholler
Part VII: Non-Viral drug Delivery System for Ovarian Cancer Therapy
33. Drug Delivery Approaches for Ovarian Cancer Therapy
Anastasia Malek
34. Polymer-Based Delivery of RNA-Based Therapeutics in Ovarian Cancer
Ulrike Weirauch, Daniela Gutsch, Sabrina Hoebel, and Achim Aigner
35. Ligand-coupled lipoprotein for ovarian cancer-specific drug delivery
Ian R. Corbin
36. Mesoporous Silicon Particles for Sustained Gene Silencing
Nafis Hasan, Aman Mann, Mauro Ferrari, and Takemi Tanaka
37. Exosomes as a Potential Tool for a Specific delivery of Functional Molecules
Irina Nazarenko, Anne-Kathleen Rupp, and Peter Altevogt
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2013
Pages: 430
Weight: 652g
Availability: Available
Subcategories: Obstetrics and Gynaecology, Oncology
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