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Main description:
Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.
Contents:
Immunoassay Techniques
Michael J. Wheeler
High-Performance Liquid Chromatography for Hormone Assay
John W. Honour
Gas Chromatography-Mass Spectrometry
John W. Honour
An Introduction to PCR Techniques
Gill Rumsby
Methods for the Investigation of Thyroid Function
Christine R. Squire
Assays for LH, FSH, and Prolactin
Michael J. Wheeler
Adrenocorticotropic Hormone and Related Peptides
Les A. Perry
Measurement of Glucocorticoids in Biological Fluids
Geoff Holder
Urinary Steroid Profiling
Norman F. Taylor
Measurement of Aldosterone in Blood
Marion L. Cawood
Measurement of Plasma Renin Activity
Marion L. Cawood
Measurement of Androgens
Michael J. Wheeler
Measurement of Gut Hormones in Plasma
Shahrad Taheri, Mohammad Ghatei, and Steve Bloom
Measurement of Melatonin and 6-Sulphatoxymelatonin
Benita Middleton
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: January, 2006
Pages: 268
Weight: 1230g
Availability: Available
Subcategories: Biochemistry
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