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DNA Vaccines

Methods and Protocols

Series: Methods in Molecular Biology

by Monica Rinaldi, Daniela Fioretti and Sandra Iurescia

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Main description:

DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines. Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease.

Contents:

Part I: DNA Vaccine Design and Enhancement

1. A Blueprint for DNA Vaccines Design

Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi

2. Enhancement of Plasmid-Mediated Transgene Expression

Daniela Fioretti, Sandra Iurescia, and Monica Rinaldi

3. Strategies for Improving DNA Vaccines Performance

Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi

4. Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies

Elisabete Borges Freitas, Ana Margarida Henriques, Miguel Fevereiro, Duarte Miguel Prazeres, and Gabriel Amaro Monteiro

5. Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies

Ronald J. Jackson, David B. Boyle, and Charani Ranasinghe

6. Development of Antibiotic-Free Selection System for Safer DNA Vaccination

Jeremy M. Luke, Aaron E. Carnes, and James A. Williams

Part II: Delivery System

7. Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin

Anita Gothelf and Julie Gehl

8. DNA Vaccination in Skin Enhanced by Electroporation

Kate E. Broderick, Amir S. Khan, and Niranjan S. Sardesai

9. Intradermal Vaccination by DNA Tattooing

Joost H. van den Berg, Koen Oosterhuis, Ton N.M. Schumacher, John B.A.G. Haanen, and Adriaan D. Bins

10. Microneedle Applications for DNA Vaccine Delivery to the Skin

Hae-yong Seok, Hyemee Suh, Sunghyun Baek, and Yeu-Chun Kim

11. Multivalent DNA-Based Vectors for DNA Vaccines Delivery

Young Hoon Roh, Kwang Lee, Jessica Jane Ye, and Dan Luo

12. Superparamagnetic Nanoparticles Delivery of DNA Vaccine

Fatin Nawwab Al-Deen, Cordelia Selomulya, Charles Ma, and Ross L. Coppel

Part III: Production, Purification, and Quality

13. Plasmid Fermentation Process for DNA Immunization Applications

Aaron E. Carnes and James A. Williams

14. Pharmaceutical Grade Large Scale Plasmid DNA Manufacturing Process

Marco Schmeer and Martin Schleef

Part IV: New Vaccine Applications

15. Protective and Therapeutic DNA Vaccination Against Allergic Diseases

Almedina Isakovic, Richard Weiss, Josef Thalhamer, and Sandra Scheiblhofer

16. Immunotherapy for Alzheimer's Disease: DNA- and Protein-Based Epitope Vaccines

Hayk Davtyan, Irina Petrushina, and Anahit Ghochikyan

17. Tetravalent DNA Vaccine Product as a Vaccine Candidate Against Dengue

Kevin R. Porter, Nimfa Teneza-Mora, and Kanakatte Raviprakash

18. DNA Vaccination as a Treatment for Chronic Kidney Disease

Yuan Min Wang and Stephen I. Alexander

PRODUCT DETAILS

ISBN-13: 9781493904099
Publisher: Springer (Humana Press Inc.)
Publication date: April, 2014
Pages: 340
Weight: 8185g
Availability: Available
Subcategories: Immunology

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