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Main description:
With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.
Contents:
Part I: Overviews of Techniques and Approaches in LC-MS-Based Proteomics
1. Approaches and Applications of Quantitative LC-MS for Proteomics and Activitomics
Pedro R. Cutillas and John F. Timms
2. Overview of Quantitative LC-MS Techniques for Proteomics and Activitomics
John F. Timms and Pedro R. Cutillas
3. Instrumentation for LC-MS/MS in Proteomics
Robert Chalkley
4. Bioinformatics for LC-MS/MS-Based Proteomics
Richard J. Jacob
5. Analysis of Post-Translational Modifications by LC-MS/MS
Hannah Johnson and Claire E. Eyers
Part II: Structural Analysis of Post-Translational Modifications
6. In-Depth Analysis of Protein Phosphorylation by Multidimensional Ion Exchange Chromatography and Mass Spectrometry
Maria P. Alcolea and Pedro R. Cutillas
7. Mapping of Phosphorylation Sites by LC-MS/MS
Bertran Gerrits and Bernd Bodenmiller
8. Analysis of Carbohydrates on Proteins by Off-Line Normal Phase Liquid Chromatography MALDI-TOF/TOF-MS/MS
Theodora Tryfona and Elaine Stephens
Part III: Techniques for Quantitative LC-MS
9. Selected Reaction Monitoring Applied to Quantitative Proteomics
Reiko Kiyonami and Bruno Domon
10. Basic Design of MRM Assays for Peptide Quantification
Andrew James and Claus Jorgensen
11. Proteome-Wide Quantitation by SILAC
Kristoffer T.G. Rigbolt and Blagoy Blagoev
12. Quantification of Proteins by iTRAQ
Richard D. Unwin
13. Quantification of Proteins by Label Free LC-MS/MS
Yishai Levin and Sabine Bahn
Part IV: Analysis of Protein Complexes and Organelles
14. Protocol for Quantitative Proteomics of Cellular Membranes and Membrane Rafts
Andrew J. Thompson and Ritchie Williamson
15. Organelle Proteomics by Label-Free and SILAC-Based Protein Correlation Profiling
Joern Dengjel, Lis Jakobsen, and Jens S. Andersen
16. Mapping Protein-Protein Interactions by Quantitative Proteomics
Joern Dengjel, Irina Kratchmarova, and Blagoy Blagoev
Part V: Analysis of Biological Fluids and Clinical Samples
17. Analysis of Serum Proteins by LC-MS/MS
Sarah Tonack, John P. Neoptolemos, and Eithne Costello
18. Urinary Proteome Profiling Using 2D-DIGE and LC-MS/MS
Mark E. Weeks
19. Analysis of Peptides in Biological Fluids by LC-MS/MS
Pedro R. Cutillas
Part VI: Novel Applications of LC-MS
20. Quantification of Protein Kinase Activities by LC-MS
Maria P. Alcolea and Pedro R. Cutillas
21. A Protocol for Top Down Proteomics Using HPLC and ETD/PTR-MS
Sarah R. Hart
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: August, 2010
Pages: 340
Weight: 1880g
Availability: Available
Subcategories: Biochemistry
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