(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.
Contents:
1. A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells
Nikko P. Torres and Grant W. Brown
2. Microscopy Techniques to Examine DNA Replication in Fission Yeast
Marc D. Green, Sarah A. Sabatinos, and Susan L. Forsburg
3. High-Resolution Analysis of Mammalian DNA Replication Units
Vadim O. Chagin, Marius Reinhart, and M. Cristina Cardoso
4. Analysing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing
Marta Bialic, Vincent Coulon, Marjorie Drac, Thierry Gostan, and Etienne Schwob
5. Measuring DNA Content by Flow Cytometry in Fission Yeast
Sarah A. Sabatinos and Susan L. Forsburg
6. Incorporation of Thymidine Analogs for Studying Replication Kinetics In Fission Yeast
Nicholas Rhind
7. Edu Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast
Nicolas Talarek, Julie Petit, Elisabeth Gueydon, and Etienne Schwob
8. Determination Of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection
Shaodong Jia, Lisette Marjavaara, Robert Buckland, Sushma Sharma, and Andrei Chabes
9. Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo
Anders R. Clausen, Jessica S. Williams, and Thomas A. Kunkel
10. Detection And Sequencing Of Okazaki Fragments In S. Cerevisiae
Duncan J. Smith, Tejas Yadav, and Lestyn Whitehouse
11. Chip-Seq To Analyze The Binding Of Replication Proteins To Chromatin
A. Zachary Ostrow, Christopher J. Viggiani, Jennifer G. Aparicio, and Oscar M. Aparicio
12. Chromatin Immunoprecipitation To Detect DNA Replication And Repair Factors
Mariana C. Gadaleta, Osamu Iwasaki, Chiaki Noguchi, Ken-Ichi Noma, and Eishi Noguchi
13. Molecular Genetic Methods To Study DNA Replication Protein Function In Haloferax Volcanii, a Model Archaeal Organism
Xavier Giroux and Stuart A. Macneill
14. Single-Molecule Observation Of Prokaryotic DNA Replication
Hylkje J. Geertsema, Karl E. Duderstadt, and Antoine M. Van Oijen
15. Analysing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast
Saed Mohebi, Sarah A. E. Lambert, and Antony M. Carr
16. Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis
Larry D. Mesner, Pieter A. Dijkwel, and Joyce L. Hamlin
17. Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes
Larry D. Mesner and Joyce L. Hamlin
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: April, 2015
Pages: 260
Weight: 7938g
Availability: Available
Subcategories: Genetics
From the same series
