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MORE ABOUT THIS BOOK
Main description:
Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.
Feature:
HPLC analysis and purification of peptides
MS/MS spectra identification of proteins
Peptide-mediated targeting of liposomes to tumor cells
Back cover:
Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.
Contents:
Peptide Characterization and Application Protocols
Preface
Contributors
Section 1: Characterization
Chapter 1: HPLC Analysis and Purification of Peptides
Colin T. Mant, Yuxin Chen, Zhe Yan, Traian V. Popa, James M. Kovacs, Janine B. Mills, Brian P. Tripet, and Robert S. Hodges
Chapter 2: Identification of Proteins Based on MS/MS Spectra and Location of Post-translational Modifications
Kathryn L. Stone, Myron Crawford, Walter McMurray, Nancy Williams, and Kenneth R. Williams
Chapter 3: Determination of Phosphorylated and O-glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature
Gary M. Hathaway
Chapter 4: Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein-Coupled Receptor
Fred Naider
Section 2: Application
Chapter 5: Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors
Janelle L. Lauer-Fields, Dmitriy Minond, Keith Brew, and Gregg B. Fields
Chapter 6: Proteolytic Profiling of the Extracellular Matrix Degradome
Diane Baronas-Lowell, Janelle L. Lauer-Fields, Mohammad Al-Ghoul, and Gregg B. Fields
Chapter 7: Å -Amyloid Protein Aggregation Inhibition
Marcus A. Etienne, Nadia J. Edwin, Jed P. Aucoin, Paul S. Russo, Robin L. McCarley, and Robert P. Hammer
Chaper 8: Radiometal-labeled Somatostatin Analogues for Applications in Cancer Imaging and Therapy
Jason S. Lewis and Carolyn J. Anderson
Chapter 9: Cell Penetrating Proline Rich Peptidomimetics
Josep Farrera-Sinfreu, Ernest Giralt, Miriam Royo, and Fernando Albericio
Chapter 10: Peptide-Mediated Targeting of Liposomes to Tumor Cells
Evonne M. Rezler, David R. Khan, Raymond Tu, Matthew Tirrell, and Gregg B. Fields
Chapter 11: Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells
Sébastien Deshayes, Federica Simeoni, May C. Morris, Gilles Divita, and FrédéricHeitz
Chaper 12: Broth Microdilution Antibacterial Assay of Peptides
Laszlo Otvos and Mare Cudic
Chapter 13: Depsipeptide Synthesis
Maciej Stawikowski and Predrag Cudic
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: May, 2007
Pages: 342
Weight: 695g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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